RRC ID 34226
著者 Michener JK, Vuilleumier S, Bringel F, Marx CJ.
タイトル Phylogeny poorly predicts the utility of a challenging horizontally transferred gene in Methylobacterium strains.
ジャーナル J Bacteriol
Abstract Horizontal gene transfer plays a crucial role in microbial evolution. While much is known about the mechanisms that determine whether physical DNA can be transferred into a new host, the factors determining the utility of the transferred genes are less clear. We have explored this issue using dichloromethane consumption in Methylobacterium strains. Methylobacterium extorquens DM4 expresses a dichloromethane dehalogenase (DcmA) that has been acquired through horizontal gene transfer and allows the strain to grow on dichloromethane as the sole carbon and energy source. We transferred the dcmA gene into six Methylobacterium strains that include both close and distant evolutionary relatives. The transconjugants varied in their ability to grow on dichloromethane, but their fitness on dichloromethane did not correlate with the phylogeny of the parental strains or with any single tested physiological factor. This work highlights an important limiting factor in horizontal gene transfer, namely, the capacity of the recipient strain to accommodate the stress and metabolic disruption resulting from the acquisition of a new enzyme or pathway. Understanding these limitations may help to rationalize historical examples of horizontal transfer and aid deliberate genetic transfers in biotechnology for metabolic engineering.
巻・号 196(11)
ページ 2101-7
公開日 2014-6-1
DOI 10.1128/JB.00034-14
PII JB.00034-14
PMID 24682326
PMC PMC4010993
MeSH Gene Expression Regulation, Bacterial / physiology* Gene Expression Regulation, Enzymologic / physiology Gene Transfer, Horizontal / physiology* Hydrogen-Ion Concentration Lyases / genetics Lyases / metabolism* Methylene Chloride / metabolism Methylobacterium / classification* Methylobacterium / genetics* Phylogeny*
IF 3.006
引用数 15
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 2831