RRC ID 30755
著者 Zhong L, Zhou W, Wang H, Ding S, Lu Q, Wen X, Peng L, Zhang L, Lu C.
タイトル Chloroplast small heat shock protein HSP21 interacts with plastid nucleoid protein pTAC5 and is essential for chloroplast development in Arabidopsis under heat stress.
ジャーナル Plant Cell
Abstract Compared with small heat shock proteins (sHSPs) in other organisms, those in plants are the most abundant and diverse. However, the molecular mechanisms by which sHSPs are involved in cell protection remain unknown. Here, we characterized the role of HSP21, a plastid nucleoid-localized sHSP, in chloroplast development under heat stress. We show that an Arabidopsis thaliana knockout mutant of HSP21 had an ivory phenotype under heat stress. Quantitative real-time RT-PCR, run-on transcription, RNA gel blot, and polysome association analyses demonstrated that HSP21 is involved in plastid-encoded RNA polymerase (PEP)-dependent transcription. We found that the plastid nucleoid protein pTAC5 was an HSP21 target. pTAC5 has a C4-type zinc finger similar to that of Escherichia coli DnaJ and zinc-dependent disulfide isomerase activity. Reduction of pTAC5 expression by RNA interference led to similar phenotypic effects as observed in hsp21. HSP21 and pTAC5 formed a complex that was associated mainly with the PEP complex. HSP21 and pTAC5 were associated with the PEP complex not only during transcription initiation, but also during elongation and termination. Our results suggest that HSP21 and pTAC5 are required for chloroplast development under heat stress by maintaining PEP function.
巻・号 25(8)
ページ 2925-43
公開日 2013-8-1
DOI 10.1105/tpc.113.111229
PII tpc.113.111229
PMID 23922206
PMC PMC3784589
MeSH Arabidopsis / drug effects Arabidopsis / genetics Arabidopsis / physiology* Arabidopsis / radiation effects Arabidopsis Proteins / chemistry Arabidopsis Proteins / metabolism* Carrier Proteins / chemistry Carrier Proteins / metabolism* Chloroplasts / drug effects Chloroplasts / genetics Chloroplasts / metabolism* Chloroplasts / radiation effects DNA, Chloroplast / metabolism Gene Expression Regulation, Plant / drug effects Gene Expression Regulation, Plant / radiation effects Genes, Plant / genetics Heat-Shock Proteins / chemistry Heat-Shock Proteins / metabolism* Heat-Shock Response* / drug effects Heat-Shock Response* / genetics Heat-Shock Response* / radiation effects Light Mutation / genetics Phenotype Plants, Genetically Modified Protein Binding / drug effects Protein Binding / radiation effects Protein Disulfide-Isomerases / metabolism Protein Structure, Tertiary Protein Transport / drug effects Protein Transport / radiation effects Seedlings / drug effects Seedlings / growth & development Seedlings / metabolism Seedlings / radiation effects Sequence Deletion Subcellular Fractions / drug effects Subcellular Fractions / metabolism Subcellular Fractions / radiation effects Zinc / pharmacology
IF 9.618
引用数 91
WOS 分野 PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pst15710 pst15714