RRC ID 47765
著者 Thagun C, Imanishi S, Kudo T, Nakabayashi R, Ohyama K, Mori T, Kawamoto K, Nakamura Y, Katayama M, Nonaka S, Matsukura C, Yano K, Ezura H, Saito K, Hashimoto T, Shoji T.
タイトル Jasmonate-Responsive ERF Transcription Factors Regulate Steroidal Glycoalkaloid Biosynthesis in Tomato.
ジャーナル Plant Cell Physiol
Abstract Steroidal glycoalkaloids (SGAs) are cholesterol-derived specialized metabolites produced in species of the Solanaceae. Here, we report that a group of jasmonate-responsive transcription factors of the ETHYLENE RESPONSE FACTOR (ERF) family (JREs) are close homologs of alkaloid regulators in Cathranthus roseus and tobacco, and regulate production of SGAs in tomato. In transgenic tomato, overexpression and dominant suppression of JRE genes caused drastic changes in SGA accumulation and in the expression of genes for metabolic enzymes involved in the multistep pathway leading to SGA biosynthesis, including the upstream mevalonate pathway. Transactivation and DNA-protein binding assays demonstrate that JRE4 activates the transcription of SGA biosynthetic genes by binding to GCC box-like elements in their promoters. These JRE-binding elements occur at significantly higher frequencies in proximal promoter regions of the genes regulated by JRE genes, supporting the conclusion that JREs mediate transcriptional co-ordination of a series of metabolic genes involved in SGA biosynthesis.
巻・号 57(5)
ページ 961-75
公開日 2016-5-1
DOI 10.1093/pcp/pcw067
PII pcw067
PMID 27084593
MeSH Alkaloids / biosynthesis Cyclopentanes / metabolism* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Ethylenes / metabolism* Gene Expression Regulation, Plant Oxylipins / metabolism* Phytosterols / biosynthesis* Plant Growth Regulators / metabolism* Plant Proteins / genetics Plant Proteins / metabolism Plants, Genetically Modified Promoter Regions, Genetic / genetics Solanum lycopersicum / genetics* Solanum lycopersicum / physiology Species Specificity Transcription Factors / genetics Transcription Factors / metabolism* Transcriptional Activation
IF 4.062
引用数 44
WOS 分野 PLANT SCIENCES CELL BIOLOGY
リソース情報
トマト TOMJPF00001