RRC ID 35532
著者 Tamura M, Kers JA, Cohen SN.
タイトル Second-site suppression of RNase E essentiality by mutation of the deaD RNA helicase in Escherichia coli.
ジャーナル J Bacteriol
Abstract Escherichia coli cells normally require RNase E activity to propagate and form colonies. Using random Tn10 insertion mutagenesis, we screened for second-site suppressor mutations that restore colony-forming ability (CFA) to E. coli cells lacking RNase E function and found mutations in three separate chromosomal loci that had this phenotype. Restoration of CFA by mutations in two of the genes identified was observed only in nutrient-poor medium, whereas the effects of mutation of the ATP-dependent RNA helicase DeaD were medium independent. Suppression of the rne mutant phenotype by inactivation of deaD was partial, as rne deaD doubly mutant bacteria had a greatly prolonged generation time and grew as filamentous chains in liquid medium. Moreover, we found that CFA restoration by deaD inactivation requires normal expression of the endogenous rng gene in doubly mutant rne deaD cells. Second-site suppression by deaD mutation was attributable specifically to ablation of the helicase activity of DeaD and was reversed by adventitious expression of RhlE or RNase R, both of which can unwind double-stranded RNA. Our results suggest a previously unsuspected role for RNA secondary structure as a determinant of RNase E essentiality.
巻・号 194(8)
ページ 1919-26
公開日 2012-4-1
DOI 10.1128/JB.06652-11
PII JB.06652-11
PMID 22328678
PMC PMC3318467
MeSH Chromosomes, Bacterial DEAD-box RNA Helicases / genetics DEAD-box RNA Helicases / metabolism* Endoribonucleases / genetics Endoribonucleases / metabolism* Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Deletion Gene Expression Regulation, Bacterial / physiology* Gene Expression Regulation, Enzymologic / physiology Mutagenesis, Insertional Plasmids / genetics
IF 3.006
引用数 18
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) ASKA library