RRC ID 20108
著者 Sekimoto H, Sone Y, Fujii T.
タイトル Regulation of expression of the genes for a sex pheromone by an inducer of the sex pheromone in the Closterium peracerosum-strigosum-littorale complex.
ジャーナル Planta
Abstract A sex pheromone, protoplast-release-inducing protein (PR-IP), of the Closterium peracerosum-strigosum-littorale complex is known to induce the release of protoplasts from mating-type minus (Mt-) cells during sexual reproduction and to have two subunit polypeptides of 19 and 42 kDa. Here, we describe the regulation mechanism for the release of the PR-IP. The sex pheromone was fractionated to yield subunits of 19 and 42 kDa, respectively, and each subunit was treated with V8 protease and with CNBr. By reference to the partial amino-acid sequences of the digested polypeptides, oligo nucleotides were synthesized and used as primers for the combined reverse transcription-polymerase chain reaction. Amplified fragments of DNA, of 130 bp in the case of the 19-kDa subunit and of 330 bp in the case of the 42-kDa subunit, were obtained, sequenced, and used as probes to identify the respective transcripts. From the results of Northern hybridization, the sizes of transcripts were estimated to be 1.2 kb for the 19-kDa subunit and 1.4 kb for the 42-kDa subunit. These transcripts appeared transiently only when mating-type plus (mt+) cells were treated with another sex pheromone (PR-IP Inducer) for more than 4 h in the light. By immunoblotting with anti-42-kDa-subunit antiserum, it was shown that PR-IP accumulated gradually in the medium but not in the mt+ cells after treatment with PR-IP inducer in the light.(ABSTRACT TRUNCATED AT 250 WORDS)
巻・号 193(1)
ページ 137-44
公開日 1994-1-1
DOI 10.1007/BF00191617
PMID 7764622
MeSH Amino Acid Sequence Base Sequence Chlorophyta / genetics* Culture Media DNA Probes DNA, Complementary Gene Expression Regulation* Genes, Plant Molecular Sequence Data Plant Proteins / genetics* Protoplasts RNA, Messenger / analysis Reproduction / genetics
IF 3.39
引用数 34
WOS 分野 PLANT SCIENCES
リソース情報
藻類 NIES-67 NIES-68