RRC ID 4806
著者 Dedeurwaerder S, Menu-Bouaouiche L, Mareck A, Lerouge P, Guerineau F.
タイトル Activity of an atypical Arabidopsis thaliana pectin methylesterase.
ジャーナル Planta
Abstract An Arabidopsis thaliana pectin methylesterase that was not predicted to contain any signaling sequence was produced in E. coli and purified using a His tag added at its N-terminus. The enzyme demethylesterified Citrus pectin with a Km of 0.86 mg/ml. The enzyme did not require salt for activity and was found to be relatively temperature-sensitive. The precipitation of enzyme-treated pectin by CaCl2 suggested that the enzyme had a blockwise mode of pectin demethylesterification. A purified kiwi (Actinidia chinensis) pectin methylesterase inhibitor had no effect on the activity of the enzyme whereas it strongly inhibited a flax pectin methylesterase. A model of the protein structure revealed that an extra amino acid sequence in this particular Arabidopsis pectin methylesterase could form a ss-strand outside the core structure, which might be preventing the inhibitor from binding the protein.
巻・号 229(2)
ページ 311-21
公開日 2009-1-1
DOI 10.1007/s00425-008-0831-0
PMID 18936961
MeSH Actinidia / enzymology Amino Acid Sequence Arabidopsis / drug effects Arabidopsis / enzymology* Arabidopsis Proteins / antagonists & inhibitors Arabidopsis Proteins / chemistry Arabidopsis Proteins / isolation & purification Arabidopsis Proteins / metabolism* Carboxylic Ester Hydrolases / antagonists & inhibitors Carboxylic Ester Hydrolases / chemistry Carboxylic Ester Hydrolases / isolation & purification Carboxylic Ester Hydrolases / metabolism* Catalytic Domain Electrophoresis, Polyacrylamide Gel Enzyme Inhibitors / pharmacology Models, Molecular Molecular Sequence Data Protein Structure, Secondary Sequence Alignment Sequence Analysis, Protein
IF 3.39
引用数 17
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda06543