RRC ID 47493
著者 Murata W, Kinpara S, Kitahara N, Yamaguchi Y, Ogita A, Tanaka T, Fujita K.
タイトル Cytoskeletal impairment during isoamyl alcohol-induced cell elongation in budding yeast.
ジャーナル Sci Rep
Abstract Isoamyl alcohol (IAA) induces pseudohyphae including cell elongation in the budding yeast Saccharomyces cerevisiae. Detailed regulation of microtubules and actin in developmental transition during cell elongation is poorly understood. Here, we show that although IAA did not affect the intracellular actin level, it reduced the levels of both α- and β-tubulins. In budding yeast, cytoplasmic microtubules are linked to actin via complexes consisting of at least Kar9, Bim1, and Myo2, and reach from the spindle pole body to the cortical attachment site at the bud tip. However, IAA did not affect migration of Myo2 to the bud tip and kept Kar9 in the interior portion of the cell. In addition, bud elongation was observed in Kar9-overexpressing cells in the absence of IAA. These results indicate that impairment of the link between cytoplasmic microtubules and actin is possibly involved in the lowered interaction of Myo2 with Kar9. Our study might explain the reason for delayed cell cycle during IAA-induced cell elongation.
巻・号 6
ページ 31127
公開日 2016-8-10
DOI 10.1038/srep31127
PII srep31127
PMID 27507042
PMC PMC4979020
MeSH Cytoskeleton / metabolism* Pentanols / metabolism* Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / metabolism* Tubulin / metabolism
IF 3.998
引用数 0
WOS 分野 CELL BIOLOGY
リソース情報
酵母 BY23323 BY24051