RRC ID 30179
著者 Tojo S, Matsunaga M, Matsumoto T, Kang CM, Yamaguchi H, Asai K, Sadaie Y, Yoshida K, Fujita Y.
タイトル Organization and expression of the Bacillus subtilis sigY operon.
ジャーナル J Biochem
Abstract We investigated the organization and expression of the Bacillus subtilis sigY operon, the first gene of which codes for sigmaY, a member of the extracytoplasmic function (ECF) family of sigma factors. The sigY operon, comprising six genes (sigY, yxlC, D, E, F, and G), was induced upon nitrogen starvation; it was continuously transcribed from the 31st base upstream of sigY to a neighboring convergent gene, yxlH, resulting in a 4.2-kb mRNA. The expression of the sigY operon was also positively autoregulated through sigmaY, suggesting that its transcription is likely to be directed by sigmaY. Deletion analysis of the sigY promoter, which was localized by primer extension, revealed the promoter region of sigY with the "-10" and "-35" sequences of CGTC and TGAACG, respectively. The latter sequence was distinct from those recognized by sigmaW, sigmaX, and sigmaM. The sigmaY-directed transcription of sigY was under negative regulation involving YxlD. sigY disruption affected sporulation induced by nitrogen starvation, but sigY induction upon nitrogen starvation was not associated with the sporulation process. The organization and function of the sigY operon are significantly conserved in several microorganisms living in adverse living environments.
巻・号 134(6)
ページ 935-46
公開日 2003-12-1
DOI 10.1093/jb/mvg225
PMID 14769884
MeSH Amino Acid Sequence Bacillus subtilis / chemistry* Bacillus subtilis / genetics Bacterial Proteins / biosynthesis* Bacterial Proteins / chemistry Bacterial Proteins / genetics Gene Expression Regulation, Bacterial* Molecular Sequence Data Operon* Sigma Factor / biosynthesis* Sigma Factor / chemistry Sigma Factor / genetics
IF 2.476
引用数 18
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ME9643
原核生物(枯草菌) MBS178 MBS179 MBS207 MBS208 MBS209 MBS210 MBS211 MBS222 MBS223 MBS225 MBS226 MBS227 MBS228 MBS229 MBS246 MBS247 MBS248 MBS249