| RRC ID |
33540
|
| 著者 |
Asai K, Yamaguchi H, Kang CM, Yoshida K, Fujita Y, Sadaie Y.
|
| タイトル |
DNA microarray analysis of Bacillus subtilis sigma factors of extracytoplasmic function family.
|
| ジャーナル |
FEMS Microbiol Lett
|
| Abstract |
Target gene candidates of the seven extracytoplasmic function (ECF) sigma factors of Bacillus subtilis have been surveyed using DNA microarray analysis of mRNA extracted from cells grown in Luria-Bertani broth, in which an ECF sigma factor gene was placed under the control of the spac promoter on multicopy plasmid pDG148 and overexpressed. The number of target candidates for each of the sigma factors varied greatly, and a total of 278 genes were selected. Interestingly, the above target gene candidates shared only one gene out of 94 target genes of the general stress sigma B that have been reported in the literature thus far. Furthermore, lacZ-fusion experiments based on the results of DNA microarray analysis indicated that each ECF sigma factor directs transcription of its own operon, with the exception of sigZ. The DNA microarray data collected in this study are available at the KEGG Expression Database web site (http://www.genome.ad.jp/kegg/expression/).
|
| 巻・号 |
220(1)
|
| ページ |
155-60
|
| 公開日 |
2003-3-14
|
| DOI |
10.1016/S0378-1097(03)00093-4
|
| PII |
S0378109703000934
|
| PMID |
12644242
|
| MeSH |
Bacillus subtilis / chemistry
Bacillus subtilis / genetics*
Bacterial Proteins / genetics*
Gene Expression Regulation, Bacterial
Genes, Bacterial
Multigene Family*
Oligonucleotide Array Sequence Analysis*
Promoter Regions, Genetic
RNA, Bacterial / genetics
RNA, Messenger / genetics
Recombinant Fusion Proteins / genetics
Sigma Factor / genetics*
Transcription, Genetic
|
| IF |
1.987
|
| 引用数 |
52
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 原核生物(枯草菌) |
MBS224
MBS605
MBS606
MBS607
MBS608
MBS609
MBS610
MBS612
MBS618
MBS619
MBS620
MBS621
MBS622
MBS623
MBS624
MBS625
MBS626
MBS627
MBS628
MBS629
MBS630 |
