RRC ID 33541
著者 Yoshida K, Yamaguchi H, Kinehara M, Ohki YH, Nakaura Y, Fujita Y.
タイトル Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
ジャーナル Mol Microbiol
Abstract Bacillus subtilis TnrA is a global regulator that responds to the availability of nitrogen sources and both activates and represses many genes during nitrogen-limited growth. In order to obtain a holistic view of the gene regulation depending on TnrA, we performed a genome-wide screening for TnrA-regulated genes associated with a TnrA box. A combination of DNA microarray hybridization and a genome-wide search for TnrA boxes allowed us to find 36 TnrA-regulated transcription units associated with a putative TnrA box. Gel retardation assaying, using probes carrying at least one putative TnrA box and the deletion derivatives of each box, indicated that 17 out of 36 transcription units were likely TnrA targets associated with the TnrA boxes, two of which (nasA and nasBCDEF) possessed a common TnrA box. The sequences of these TnrA boxes contained a consensus one, TGTNANAWWWTMTNACA. The TnrA targets detected in this study were nrgAB, pucJKLM, glnQHMP, nasDEF, oppABCDF, nasA, nasBCDEF and ywrD for positive regulation, and gltAB, pel, ywdIJK, yycCB, yttA, yxkC, ywlFG, yodF and alsT for negative regulation, nrgAB and gltAB being well-studied TnrA targets. It was unexpected that the negatively regulated TnrA targets were as many as the positively regulated targets. The physiological role of the TnrA regulon is discussed.
巻・号 49(1)
ページ 157-65
公開日 2003-7-1
DOI 10.1046/j.1365-2958.2003.03567.x
PII 3567
PMID 12823818
MeSH Amino Acid Motifs Bacillus subtilis / genetics* Bacillus subtilis / metabolism Bacterial Proteins / genetics Bacterial Proteins / metabolism* Gene Expression Profiling* Gene Expression Regulation, Bacterial* Genome, Bacterial Oligonucleotide Array Sequence Analysis Regulatory Sequences, Nucleic Acid* Repressor Proteins / genetics Repressor Proteins / metabolism* Transcription Factors / genetics Transcription Factors / metabolism* Transcription, Genetic
IF 3.418
引用数 67
WOS 分野 MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(枯草菌) MBS220 MBS221