RRC ID 33563
著者 Hirooka K, Danjo Y, Hanano Y, Kunikane S, Matsuoka H, Tojo S, Fujita Y.
タイトル Regulation of the Bacillus subtilis divergent yetL and yetM genes by a transcriptional repressor, YetL, in response to flavonoids.
ジャーナル J Bacteriol
Abstract DNA microarray analysis revealed that transcription of the Bacillus subtilis yetM gene encoding a putative flavin adenine dinucleotide-dependent monooxygenase was triggered by certain flavonoids during culture and was derepressed by disruption of the yetL gene in the opposite orientation situated immediately upstream of yetM, which encodes a putative MarR family transcriptional regulator. In vitro analyses, including DNase I footprinting and gel retardation analysis, indicated that YetL binds specifically to corresponding single sites in the divergent yetL and yetM promoter regions, with higher affinity to the yetM region; the former region overlaps the Shine-Dalgarno sequence of yetL, and the latter region contains a perfect 18-bp palindromic sequence (TAGTTAGGCGCCTAACTA). In vitro gel retardation and in vivo lacZ fusion analyses indicated that some flavonoids (kaempferol, apigenin, and luteolin) effectively inhibit YetL binding to the yetM cis sequence, but quercetin, galangin, and chrysin do not inhibit this binding, implying that the 4-hydroxyl group on the B-ring of the flavone structure is indispensable for this inhibition and that the coexistence of the 3-hydroxyl groups on the B- and C-rings does not allow antagonism of YetL.
巻・号 191(11)
ページ 3685-97
公開日 2009-6-1
DOI 10.1128/JB.00202-09
PII JB.00202-09
PMID 19329649
PMC PMC2681910
MeSH Apigenin / pharmacology Bacillus subtilis / drug effects* Bacillus subtilis / genetics* Bacillus subtilis / metabolism Bacterial Proteins / genetics Bacterial Proteins / metabolism* DNA Footprinting Flavonoids / pharmacology* Gene Expression Regulation, Bacterial / drug effects* Gene Expression Regulation, Bacterial / genetics Kaempferols / pharmacology Luteolin / pharmacology Oligonucleotide Array Sequence Analysis Promoter Regions, Genetic / genetics Protein Binding
IF 3.006
引用数 14
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(枯草菌) MBS324 MBS325 MBS326 MBS327 MBS328