RRC ID 33577
著者 Yano K, Wada T, Suzuki S, Tagami K, Matsumoto T, Shiwa Y, Ishige T, Kawaguchi Y, Masuda K, Akanuma G, Nanamiya H, Niki H, Yoshikawa H, Kawamura F.
タイトル Multiple rRNA operons are essential for efficient cell growth and sporulation as well as outgrowth in Bacillus subtilis.
ジャーナル Microbiology (Reading)
Abstract The number of copies of rRNA (rrn) operons in a bacterial genome differs greatly among bacterial species. Here we examined the phenotypic effects of variations in the number of copies of rRNA genes in the genome of Bacillus subtilis by analysis of eight mutant strains constructed to carry from two to nine copies of the rrn operon. We found that a decrease in the number of copies from ten to one increased the doubling time, and decreased the sporulation frequency and motility. The maximum levels for transformation activity were similar among the strains, although the competence development was significantly delayed in the strain with a single rrn operon. Normal sporulation only occurred if more than four copies of the rrn operon were present, although ten copies were needed for vegetative growth after germination of the spores. This behaviour was seen even though the intracellular level of ribosomes was similar among strains with four to ten copies of the rrn operon. Furthermore, ten copies of the rrn operon were needed for the highest swarming activity. We also constructed 21 strains that carried all possible combinations of two copies of the rrn operons, and found that these showed a range of growth rates and sporulation frequencies that all fell between those recorded for strains with one or three copies of the rrn operon. The results suggested that the copy number of the rrn operon has a major influence on cellular processes such as growth rate and sporulation frequency.
巻・号 159(Pt 11)
ページ 2225-2236
公開日 2013-11-1
DOI 10.1099/mic.0.067025-0
PMID 23970567
MeSH Bacillus subtilis / genetics* Bacillus subtilis / growth & development* Bacillus subtilis / physiology Cell Division DNA Transformation Competence Gene Dosage Genes, Essential Locomotion Mutation Spores, Bacterial / genetics* Spores, Bacterial / growth & development* Spores, Bacterial / physiology Transformation, Bacterial rRNA Operon*
IF 2.138
引用数 35
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(枯草菌) MBS375 MBS376 MBS377 MBS378 MBS379 MBS380 MBS381 MBS382 MBS383 MBS384 MBS385 MBS386 MBS387 MBS388 MBS389 MBS390 MBS391 MBS392 MBS393 MBS394 MBS395 MBS396 MBS397 MBS398 MBS399 MBS400 MBS401 MBS402 MBS403