RRC ID |
33577
|
著者 |
Yano K, Wada T, Suzuki S, Tagami K, Matsumoto T, Shiwa Y, Ishige T, Kawaguchi Y, Masuda K, Akanuma G, Nanamiya H, Niki H, Yoshikawa H, Kawamura F.
|
タイトル |
Multiple rRNA operons are essential for efficient cell growth and sporulation as well as outgrowth in Bacillus subtilis.
|
ジャーナル |
Microbiology (Reading)
|
Abstract |
The number of copies of rRNA (rrn) operons in a bacterial genome differs greatly among bacterial species. Here we examined the phenotypic effects of variations in the number of copies of rRNA genes in the genome of Bacillus subtilis by analysis of eight mutant strains constructed to carry from two to nine copies of the rrn operon. We found that a decrease in the number of copies from ten to one increased the doubling time, and decreased the sporulation frequency and motility. The maximum levels for transformation activity were similar among the strains, although the competence development was significantly delayed in the strain with a single rrn operon. Normal sporulation only occurred if more than four copies of the rrn operon were present, although ten copies were needed for vegetative growth after germination of the spores. This behaviour was seen even though the intracellular level of ribosomes was similar among strains with four to ten copies of the rrn operon. Furthermore, ten copies of the rrn operon were needed for the highest swarming activity. We also constructed 21 strains that carried all possible combinations of two copies of the rrn operons, and found that these showed a range of growth rates and sporulation frequencies that all fell between those recorded for strains with one or three copies of the rrn operon. The results suggested that the copy number of the rrn operon has a major influence on cellular processes such as growth rate and sporulation frequency.
|
巻・号 |
159(Pt 11)
|
ページ |
2225-2236
|
公開日 |
2013-11-1
|
DOI |
10.1099/mic.0.067025-0
|
PMID |
23970567
|
MeSH |
Bacillus subtilis / genetics*
Bacillus subtilis / growth & development*
Bacillus subtilis / physiology
Cell Division
DNA Transformation Competence
Gene Dosage
Genes, Essential
Locomotion
Mutation
Spores, Bacterial / genetics*
Spores, Bacterial / growth & development*
Spores, Bacterial / physiology
Transformation, Bacterial
rRNA Operon*
|
IF |
2.138
|
引用数 |
35
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
原核生物(枯草菌) |
MBS375
MBS376
MBS377
MBS378
MBS379
MBS380
MBS381
MBS382
MBS383
MBS384
MBS385
MBS386
MBS387
MBS388
MBS389
MBS390
MBS391
MBS392
MBS393
MBS394
MBS395
MBS396
MBS397
MBS398
MBS399
MBS400
MBS401
MBS402
MBS403 |