RRC ID 33601
著者 Morimoto T, Ara K, Ozaki K, Ogasawara N.
タイトル A simple method for introducing marker-free deletions in the Bacillus subtilis genome.
ジャーナル Methods Mol Biol
Abstract A genetic tool for introducing marker-free deletions is essential for multiple manipulations of genomes. We have developed a simple and efficient method for creating marker-free deletion mutants of Bacillus subtilis through transformation with recombinant PCR products, using the Escherichia coli mazF gene encoding an endoribonuclease that cleaves free mRNAs as a counterselection tool.
巻・号 765
ページ 345-58
公開日 2011-1-1
DOI 10.1007/978-1-61779-197-0_20
PMID 21815102
MeSH Bacillus subtilis / genetics* DNA, Recombinant / genetics* Endoribonucleases / genetics Endoribonucleases / metabolism Escherichia coli / enzymology Escherichia coli / genetics Gene Deletion* Genetic Markers / genetics Genome, Bacterial / genetics* Polymerase Chain Reaction Transformation, Bacterial / genetics*
引用数 6
WOS 分野 BIOCHEMICAL RESEARCH METHODS GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(枯草菌) TMO310 TMO311