RRC ID |
21551
|
著者 |
International Barley Genome Sequencing Consortium, Mayer KF, Waugh R, Brown JW, Schulman A, Langridge P, Platzer M, Fincher GB, Muehlbauer GJ, Sato K, Close TJ, Wise RP, Stein N.
|
タイトル |
A physical, genetic and functional sequence assembly of the barley genome.
|
ジャーナル |
Nature
|
Abstract |
Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.
|
巻・号 |
491(7426)
|
ページ |
711-6
|
公開日 |
2012-11-29
|
DOI |
10.1038/nature11543
|
PII |
nature11543
|
PMID |
23075845
|
MeSH |
Alternative Splicing / genetics
Codon, Nonsense / genetics
Crops, Agricultural / genetics
Evolution, Molecular
Gene Expression Regulation, Plant
Genes, Plant / genetics
Genome, Plant / genetics*
Genomics
Hordeum / classification
Hordeum / genetics*
Molecular Sequence Annotation
Physical Chromosome Mapping
Polymorphism, Single Nucleotide / genetics
Repetitive Sequences, Nucleic Acid / genetics
Sequence Analysis, DNA*
Transcriptome / genetics
|
IF |
42.779
|
引用数 |
887
|
リソース情報 |
オオムギ |
Full length cDNA
Haruna Nijo |