| RRC ID |
47946
|
| 著者 |
Aliyeva-Schnorr L, Stein N, Houben A.
|
| タイトル |
Collinearity of homoeologous group 3 chromosomes in the genus Hordeum and Secale cereale as revealed by 3H-derived FISH analysis.
|
| ジャーナル |
Chromosome Res
|
| Abstract |
Crop wild relatives are considered as important genetic resources of allelic diversity for domesticated crop species. Their utilization in breeding programs, however, is often limited due to crossing barriers and genome incompatibilities. Wild relatives of barley possess attractive properties and hence allelic diversity for adapting barley better to changing environmental conditions. Therefore, gaining a better knowledge about genomic synteny between cultivated barley and wild relatives of the same genus is an important task. To visualize genomic collinearity in related species, 22 genomic single-copy and 14 complementary DNA (cDNA) chromosome 3H-specific probes were mapped to the chromosomes of Hordeum bulbosum, Hordeum marinum, Hordeum pubiflorum, Hordeum murinum, and Secale cereale by fluorescent in situ hybridization (FISH). Most probes showed reliable signals confirming homoeology between cultivated barley and related species. Differences in order and position of FISH markers demonstrated sequence movements or small-scale chromosomal rearrangements within genus Hordeum and confirmed interchromosomal rearrangements between barley and rye. Comparison between repeat-free genomic and cDNA probes showed that gene-containing single-copy genomic DNA (gDNA) probes are performing more reliably for FISH-based analysis of synteny.
|
| 巻・号 |
24(2)
|
| ページ |
231-42
|
| 公開日 |
2016-5-1
|
| DOI |
10.1007/s10577-016-9518-8
|
| PII |
10.1007/s10577-016-9518-8
|
| PMID |
26883649
|
| MeSH |
Base Sequence
Chromosome Mapping / methods*
Chromosomes, Plant / genetics*
DNA Probes / genetics
DNA, Plant / genetics*
Expressed Sequence Tags
Hordeum / genetics*
In Situ Hybridization, Fluorescence
Secale / genetics*
Synteny / genetics*
|
| IF |
3.413
|
| 引用数 |
10
|
|
WOS 分野
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| オオムギ |
FLcDNA:
AK251893
AK248957
AK251014
AK251696
AK249216
AK336104
AK248727
AK248230
AK251227 |
