| RRC ID |
21690
|
| 著者 |
Kuno S, Yoshida T, Kamikawa R, Hosoda N, Sako Y.
|
| タイトル |
The distribution of a phage-related insertion sequence element in the cyanobacterium, Microcystis aeruginosa.
|
| ジャーナル |
Microbes Environ
|
| Abstract |
The cyanophage Ma-LMM01, specifically-infecting Microcystis aeruginosa, has an insertion sequence (IS) element that we named IS607-cp showing high nucleotide similarity to a counterpart in the genome of the cyanobacterium Cyanothece sp. We tested 21 strains of M. aeruginosa for the presence of IS607-cp using PCR and detected the element in strains NIES90, NIES112, NIES604, and RM6. Thermal asymmetric interlaced PCR (TAIL-PCR) revealed each of these strains has multiple copies of IS607-cp. Some of the ISs were classified into three types based on their inserted positions; IS607-cp-1 is common in strains NIES90, NIES112 and NIES604, whereas IS607-cp-2 and IS607-cp-3 are specific to strains NIES90 and RM6, respectively. This multiplicity may reflect the replicative transposition of IS607-cp. The sequence of IS607-cp in Ma-LMM01 showed robust affinity to those found in M. aeruginosa and Cyanothece spp. in a phylogenetic tree inferred from counterparts of various bacteria. This suggests the transfer of IS607-cp between the cyanobacterium and its cyanophage. We discuss the potential role of Ma-LMM01-related phages as donors of IS elements that may mediate the transfer of IS607-cp; and thereby partially contribute to the genome plasticity of M. aeruginosa.
|
| 巻・号 |
25(4)
|
| ページ |
295-301
|
| 公開日 |
2010-1-1
|
| DOI |
10.1264/jsme2.me10125
|
| PII |
JST.JSTAGE/jsme2/ME10125
|
| PMID |
21576885
|
| MeSH |
Bacteriophages / genetics
Bacteriophages / physiology*
Base Sequence
DNA Transposable Elements*
Microcystis / classification
Microcystis / genetics*
Microcystis / virology*
Molecular Sequence Data
Mutagenesis, Insertional
Phylogeny
|
| IF |
2.575
|
| 引用数 |
11
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 藻類 |
NIES-90
NIES-91
NIES-98
NIES-99
NIES-101
NIES-102
NIES-112
NIES-604
NIES-1058
NIES-1067
NIES-1070
NIES-1086
NIES-1098
NIES-1105
NIES-843
NIES-298 |
