RRC ID 34198
著者 Sakai M, Hosoda A, Ogura K, Ikenaga M.
タイトル The growth of Steroidobacter agariperforans sp. nov., a novel agar-degrading bacterium isolated from soil, is enhanced by the diffusible metabolites produced by bacteria belonging to Rhizobiales.
ジャーナル Microbes Environ
Abstract An agar-degrading bacterium was isolated from soil collected in a vegetable cropping field. The growth of this isolate was enhanced by supplying culture supernatants of bacteria belonging to the order Rhizobiales. Phylogenetic analysis based on 16S rRNA gene sequences indicated the novel bacterium, strain KA5-B(T), belonged to the genus Steroidobacter in Gammaproteobacteria, but differed from its closest relative, Steroidobacter denitrificans FS(T), at the species level with 96.5% similarity. Strain KA5-B(T) was strictly aerobic, Gram-negative, non-motile, non-spore forming, and had a straight to slightly curved rod shape. Cytochrome oxidase and catalase activities were positive. The strain grew on media containing culture supernatants in a temperature range of 15-37°C and between pH 4.5 and 9.0, with optimal growth occurring at 30°C and pH 6.0-8.0. No growth occurred at 10 or 42°C or at NaCl concentrations more than 3% (w/v). The main cellular fatty acids were iso-C15:0, C16:1ω7c, and iso-C17:1ω9c. The main quinone was ubiquinone-8 and DNA G+C content was 62.9 mol%. In contrast, strain FS(T) was motile, did not grow on the agar plate, and its dominant cellular fatty acids were C15:0 and C17:1ω8c. Based on its phylogenetic and phenotypic properties, strain KA5-B(T) (JCM 18477(T) = KCTC 32107(T)) represents a novel species in genus Steroidobacter, for which the name Steroidobacter agariperforans sp. nov. is proposed.
巻・号 29(1)
ページ 89-95
公開日 2014-1-1
DOI 10.1264/jsme2.me13169
PII DN/JST.JSTAGE/jsme2/ME13169
PMID 24621511
PMC PMC4041242
MeSH Agar / metabolism* Alphaproteobacteria / classification Alphaproteobacteria / growth & development* Alphaproteobacteria / isolation & purification* Alphaproteobacteria / metabolism Bacterial Typing Techniques Culture Media / chemistry Culture Media / metabolism Fatty Acids / chemistry Fatty Acids / metabolism Molecular Sequence Data Phylogeny Soil Microbiology*
IF 2.575
引用数 14
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 18477