RRC ID 29629
著者 Kakimoto M, Ishikawa T, Miyagi A, Saito K, Miyazaki M, Asaeda T, Yamaguchi M, Uchimiya H, Kawai-Yamada M.
タイトル Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeata.
ジャーナル J Plant Physiol
Abstract A volatile metabolite, 2-methylisoborneol (2-MIB), causes an unpleasant taste and odor in tap water. Some filamentous cyanobacteria produce 2-MIB via a two-step biosynthetic pathway: methylation of geranyl diphosphate (GPP) by methyl transferase (GPPMT), followed by the cyclization of methyl-GPP by monoterpene cyclase (MIBS). We isolated the genes encoding GPPMT and MIBS from Pseudanabaena galeata, a filamentous cyanobacterium known to be a major causal organism of 2-MIB production in Japanese lakes. The predicted amino acid sequence showed high similarity with that of Pseudanabaena limnetica (96% identity in GPPMT and 97% identity in MIBS). P. galeata was cultured at different temperatures to examine the effect of growth conditions on the production of 2-MIB and major metabolites. Gas chromatograph-mass spectrometry (GC-MS) measurements showed higher accumulation of 2-MIB at 30 °C than at 4 °C or 20 °C after 24 h of culture. Real-time-RT PCR analysis showed that the expression levels of the genes encoding GPPMT and MIBS decreased at 4 °C and increased at 30 °C, compared with at 20 °C. Furthermore, metabolite analysis showed dramatic changes in primary metabolite concentrations in cyanobacteria grown at different temperatures. The data indicate that changes in carbon flow in the TCA cycle affect 2-MIB biosynthesis at higher temperatures.
巻・号 171(3-4)
ページ 292-300
公開日 2014-2-15
DOI 10.1016/j.jplph.2013.09.005
PII S0176-1617(13)00360-X
PMID 24140001
MeSH Camphanes / metabolism Cyanobacteria / genetics* Cyanobacteria / metabolism* Gene Expression Regulation, Bacterial* Metabolic Networks and Pathways / physiology* Temperature*
IF 3.013
引用数 18
WOS 分野 PLANT SCIENCES
リソース情報
藻類 NIES-512