RRC ID 31001
著者 Chungopast S, Thapanapongworakul P, Matsuura H, Van Dao T, Asahi T, Tada K, Tajima S, Nomura M.
タイトル Glutamine synthetase I-deficiency in Mesorhizobium loti differentially affects nodule development and activity in Lotus japonicus.
ジャーナル J Plant Physiol
Abstract In this study, we focused on the effect of glutamine synthetase (GSI) activity in Mesorhizobium loti on the symbiosis between the host plant, Lotus japonicus, and the bacteroids. We used a signature-tagged mutant of M. loti (STM30) with a transposon inserted into the GSI (mll0343) gene. The L. japonicus plants inoculated with STM30 had significantly more nodules, and the occurrence of senesced nodules was much higher than in plants inoculated with the wild-type. The acetylene reduction activity (ARA) per nodule inoculated with STM30 was lowered compared to the control. Also, the concentration of chlorophyll, glutamine, and asparagine in leaves of STM30-infected plants was found to be reduced. Taken together, these data demonstrate that a GSI deficiency in M. loti differentially affects legume-rhizobia symbiosis by modifying nodule development and metabolic processes.
巻・号 171(5)
ページ 104-8
公開日 2014-3-1
DOI 10.1016/j.jplph.2013.10.015
PII S0176-1617(13)00424-0
PMID 24484964
MeSH Glutamate-Ammonia Ligase / deficiency Glutamate-Ammonia Ligase / genetics* Lotus / metabolism* Lotus / microbiology* Mesorhizobium / genetics Mesorhizobium / metabolism Mesorhizobium / physiology* Nitrogen Fixation* Plant Roots / metabolism Plant Roots / microbiology Real-Time Polymerase Chain Reaction Symbiosis*
IF 3.013
引用数 8
WOS 分野 PLANT SCIENCES
リソース情報
ミヤコグサ・ダイズ STM clone ID 10T05g06