| RRC ID |
34113
|
| 著者 |
Doi Y, Shimizu M, Fujita T, Nakamura A, Takizawa N, Takaya N.
|
| タイトル |
Achromobacter denitrificans strain YD35 pyruvate dehydrogenase controls NADH production to allow tolerance to extremely high nitrite levels.
|
| ジャーナル |
Appl Environ Microbiol
|
| Abstract |
We identified the extremely nitrite-tolerant bacterium Achromobacter denitrificans YD35 that can grow in complex medium containing 100 mM nitrite (NO2(-)) under aerobic conditions. Nitrite induced global proteomic changes and upregulated tricarboxylate (TCA) cycle enzymes as well as antioxidant proteins in YD35. Transposon mutagenesis generated NO2(-)-hypersensitive mutants of YD35 that had mutations at genes for aconitate hydratase and α-ketoglutarate dehydrogenase in the TCA cycle and a pyruvate dehydrogenase (Pdh) E1 component, indicating the importance of TCA cycle metabolism to NO2(-) tolerance. A mutant in which the pdh gene cluster was disrupted (Δpdh mutant) could not grow in the presence of 100 mM NO2(-). Nitrite decreased the cellular NADH/NAD(+) ratio and the cellular ATP level. These defects were more severe in the Δpdh mutant, indicating that Pdh contributes to upregulating cellular NADH and ATP and NO2(-)-tolerant growth. Exogenous acetate, which generates acetyl coenzyme A and then is metabolized by the TCA cycle, compensated for these defects caused by disruption of the pdh gene cluster and those caused by NO2(-). These findings demonstrate a link between NO2(-) tolerance and pyruvate/acetate metabolism through the TCA cycle. The TCA cycle mechanism in YD35 enhances NADH production, and we consider that this contributes to a novel NO2(-)-tolerating mechanism in this strain.
|
| 巻・号 |
80(6)
|
| ページ |
1910-8
|
| 公開日 |
2014-3-1
|
| DOI |
10.1128/AEM.03316-13
|
| PII |
AEM.03316-13
|
| PMID |
24413603
|
| PMC |
PMC3957653
|
| MeSH |
Achromobacter denitrificans / drug effects*
Achromobacter denitrificans / enzymology*
Achromobacter denitrificans / growth & development
Aerobiosis
DNA Transposable Elements
Drug Resistance, Bacterial*
Gene Deletion
Molecular Sequence Data
Mutagenesis, Insertional
NAD / metabolism*
Nitrites / metabolism*
Nitrites / toxicity*
Pyruvate Dehydrogenase (Lipoamide) / metabolism*
Sequence Analysis, DNA
|
| IF |
4.016
|
| 引用数 |
6
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 19165 |
