RRC ID 34296
著者 Mora M, Bellack A, Ugele M, Hopf J, Wirth R.
タイトル The temperature gradient-forming device, an accessory unit for normal light microscopes to study the biology of hyperthermophilic microorganisms.
ジャーナル Appl Environ Microbiol
Abstract To date, the behavior of hyperthermophilic microorganisms in their biotope has been studied only to a limited degree; this is especially true for motility. One reason for this lack of knowledge is the requirement for high-temperature microscopy-combined, in most cases, with the need for observations under strictly anaerobic conditions-for such studies. We have developed a custom-made, low-budget device that, for the first time, allows analyses in temperature gradients up to 40°C over a distance of just 2 cm (a biotope-relevant distance) with heating rates up to ∼5°C/s. Our temperature gradient-forming device can convert any upright light microscope into one that works at temperatures as high as 110°C. Data obtained by use of this apparatus show how very well hyperthermophiles are adapted to their biotope: they can react within seconds to elevated temperatures by starting motility-even after 9 months of storage in the cold. Using the temperature gradient-forming device, we determined the temperature ranges for swimming, and the swimming speeds, of 15 selected species of the genus Thermococcus within a few months, related these findings to the presence of cell surface appendages, and obtained the first evidence for thermotaxis in Archaea.
巻・号 80(15)
ページ 4764-70
公開日 2014-8-1
DOI 10.1128/AEM.00984-14
PII AEM.00984-14
PMID 24858087
PMC PMC4148812
MeSH Equipment Design Hot Temperature Microscopy / instrumentation Microscopy / methods* Thermococcus / chemistry Thermococcus / classification Thermococcus / cytology*
IF 4.016
引用数 6
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 12380