RRC ID 34334
著者 Bao Z, Okubo T, Kubota K, Kasahara Y, Tsurumaru H, Anda M, Ikeda S, Minamisawa K.
タイトル Metaproteomic identification of diazotrophic methanotrophs and their localization in root tissues of field-grown rice plants.
ジャーナル Appl Environ Microbiol
Abstract In a previous study by our group, CH4 oxidation and N2 fixation were simultaneously activated in the roots of wild-type rice plants in a paddy field with no N input; both processes are likely controlled by a rice gene for microbial symbiosis. The present study examined which microorganisms in rice roots were responsible for CH4 oxidation and N2 fixation under the field conditions. Metaproteomic analysis of root-associated bacteria from field-grown rice (Oryza sativa Nipponbare) revealed that nitrogenase complex-containing nitrogenase reductase (NifH) and the alpha subunit (NifD) and beta subunit (NifK) of dinitrogenase were mainly derived from type II methanotrophic bacteria of the family Methylocystaceae, including Methylosinus spp. Minor nitrogenase proteins such as Methylocella, Bradyrhizobium, Rhodopseudomonas, and Anaeromyxobacter were also detected. Methane monooxygenase proteins (PmoCBA and MmoXYZCBG) were detected in the same bacterial group of the Methylocystaceae. Because these results indicated that Methylocystaceae members mediate both CH4 oxidation and N2 fixation, we examined their localization in rice tissues by using catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH). The methanotrophs were localized around the epidermal cells and vascular cylinder in the root tissues of the field-grown rice plants. Our metaproteomics and CARD-FISH results suggest that CH4 oxidation and N2 fixation are performed mainly by type II methanotrophs of the Methylocystaceae, including Methylosinus spp., inhabiting the vascular bundles and epidermal cells of rice roots.
巻・号 80(16)
ページ 5043-52
公開日 2014-8-1
DOI 10.1128/AEM.00969-14
PII AEM.00969-14
PMID 24928870
PMC PMC4135783
MeSH Autotrophic Processes Bacteria / classification Bacteria / genetics Bacteria / isolation & purification* Bacteria / metabolism* Bacterial Proteins / genetics Bacterial Proteins / metabolism Methane / metabolism* Molecular Sequence Data Nitrogen Fixation Nitrogenase / genetics Nitrogenase / metabolism Oryza / growth & development Oryza / microbiology* Phylogeny Plant Roots / growth & development Plant Roots / microbiology Proteomics* Soil Microbiology
IF 4.016
引用数 37
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 20098