RRC ID |
37036
|
著者 |
Takei T, Aokawa R, Shigemitsu T, Kawakami K, Yoshida M.
|
タイトル |
Fabrication of uniformly cell-laden porous scaffolds using a gas-in-liquid templating technique.
|
ジャーナル |
J Biosci Bioeng
|
Abstract |
Design of porous scaffolds in tissue engineering field was challenging. Uniform immobilization of cells in the scaffolds with high porosity was essential for homogeneous tissue formation. The present study was aimed at fabricating uniformly cell-laden porous scaffolds with porosity >74% using the gas-in-liquid foam templating technique. To this end, we used gelatin, microbial transglutaminase and argon gas as a scaffold material, cross-linker of the protein and porogen of scaffold, respectively. We confirmed that a porosity of >74% could be achieved by increasing the gas volume delivered to a gelatin solution. Pore size in the scaffold could be controlled by stirring speed, stirring time and the pore size of the filter through which the gas passed. The foaming technique enabled us to uniformly immobilize a human hepatoblastoma cell line in scaffold. Engraftment efficiency of the cell line entrapped within the scaffold in nude mice was higher than that of cells in free-form. These results showed that the uniformly cell-laden porous scaffolds were promising for tissue engineering.
|
巻・号 |
120(5)
|
ページ |
577-81
|
公開日 |
2015-11-1
|
DOI |
10.1016/j.jbiosc.2015.03.017
|
PII |
S1389-1723(15)00144-9
|
PMID |
25912452
|
MeSH |
Animals
Argon*
Cell Line
Cell Line, Tumor
Cells, Immobilized*
Gelatin
Hepatoblastoma / pathology
Humans
Male
Mice
Mice, Nude
Porosity
Tissue Engineering / methods*
Tissue Scaffolds / chemistry*
Transglutaminases / metabolism
|
IF |
2.366
|
引用数 |
5
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
Hep G2(RCB1886) |