RRC ID 30773
著者 Tanaka K, Hayashi K, Natsume M, Kamiya Y, Sakakibara H, Kawaide H, Kasahara H.
タイトル UGT74D1 catalyzes the glucosylation of 2-oxindole-3-acetic acid in the auxin metabolic pathway in Arabidopsis.
ジャーナル Plant Cell Physiol
Abstract IAA is a naturally occurring auxin that plays a crucial role in the regulation of plant growth and development. The endogenous concentration of IAA is spatiotemporally regulated by biosynthesis, transport and its inactivation in plants. Previous studies have shown that the metabolism of IAA to 2-oxindole-3-acetic acid (OxIAA) and OxIAA-glucoside (OxIAA-Glc) may play an important role in IAA homeostasis, but the genes involved in this metabolic pathway are still unknown. In this study, we show that UGT74D1 catalyzes the glucosylation of OxIAA in Arabidopsis. By screening yeasts transformed with Arabidopsis UDP-glycosyltransferase (UGT) genes, we found that OxIAA-Glc accumulates in the culture media of yeasts expressing UGT74D1 in the presence of OxIAA. Further, we showed that UGT74D1 expressed in Escherichia coli converts OxIAA to OxIAA-Glc. The endogenous concentration of OxIAA-Glc decreased by 85% while that of OxIAA increased 2.5-fold in ugt74d1-deficient mutants, indicating the major role of UGT74D1 in OxIAA metabolism. Moreover, the induction of UGT74D1 markedly increased the level of OxIAA-Glc and loss of root gravitropism. These results indicate that UGT74D1 catalyzes a committed step in the OxIAA-dependent IAA metabolic pathway in Arabidopsis.
巻・号 55(1)
ページ 218-28
公開日 2014-1-1
DOI 10.1093/pcp/pct173
PII pct173
PMID 24285754
PMC PMC3894777
MeSH Arabidopsis / drug effects Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Biocatalysis* / drug effects Biosynthetic Pathways / drug effects Biosynthetic Pathways / genetics Enzyme Induction / drug effects Gene Expression Regulation, Plant / drug effects Genes, Plant / genetics Glucosyltransferases / genetics Glucosyltransferases / metabolism* Glycosylation / drug effects Glycosyltransferases / biosynthesis Gravitropism / drug effects Indoleacetic Acids / chemistry Indoleacetic Acids / metabolism* Indoleacetic Acids / pharmacology Kinetics Metabolic Networks and Pathways* / drug effects Metabolic Networks and Pathways* / genetics Oxindoles Plant Roots / drug effects Plant Roots / genetics Plant Roots / physiology
IF 4.062
引用数 36
WOS 分野 PLANT SCIENCES CELL BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda01666 pda04758 pda04986 pda05237 pda07420 pda08060 pda09424 pda11460 pda15474 pda20336 pda00614 pda04176 pda12406