RRC ID 53110
著者 Htet AN, Noguchi M, Ninomiya K, Tsuge Y, Kuroda K, Kajita S, Masai E, Katayama Y, Shikinaka K, Otsuka Y, Nakamura M, Honda R, Takahashi K.
タイトル Application of microalgae hydrolysate as a fermentation medium for microbial production of 2-pyrone 4,6-dicarboxylic acid.
ジャーナル J Biosci Bioeng
Abstract Actual biomass of microalgae was tested as a fermentation substrate for microbial production of 2-pyrone 4,6-dicarboxylic acid (PDC). Acid-hydrolyzed green microalgae Chlorella emersonii (algae hydrolysate) was diluted to adjust the glucose concentration to 2 g/L and supplemented with the nutrients of Luria-Bertani (LB) medium (tryptone 10 g/L and yeast extract 5 g/L). When the algae hydrolysate was used as a fermentation source for recombinant Escherichia coli producing PDC, 0.43 g/L PDC was produced with a yield of 20.1% (mol PDC/mol glucose), whereas 0.19 g/L PDC was produced with a yield of 8.6% when LB medium supplemented with glucose was used. To evaluate the potential of algae hydrolysate alone as a fermentation medium for E. coli growth and PDC production, the nutrients of LB medium were reduced from the algae hydrolysate medium. Interestingly, 0.17 g/L PDC was produced even without additional nutrient, which was comparable to the case using pure glucose medium with nutrients of LB medium. When using a high concentration of hydrolysate without additional nutrients, 1.22 g/L PDC was produced after a 24-h cultivation with the yield of 16.1%. Overall, C. emersonii has high potential as cost-effective fermentation substrate for the microbial production of PDC.
巻・号 125(6)
ページ 717-722
公開日 2018-6-1
DOI 10.1016/j.jbiosc.2017.12.026
PII S1389-1723(17)31105-2
PMID 29395960
MeSH Biomass Carboxylic Ester Hydrolases / metabolism Chlorella / enzymology Chlorella / growth & development Chlorella / metabolism* Escherichia coli / genetics Escherichia coli / metabolism* Fermentation* Glucose / metabolism Hydrolysis Microalgae / enzymology Microalgae / growth & development Microalgae / metabolism* Organisms, Genetically Modified Pyrones / metabolism*
IF 2.366
引用数 6
リソース情報
藻類 NIES-2151