RRC ID |
33726
|
著者 |
Habib AG, Masuda K, Yukawa M, Tsuchiya E, Ueno M.
|
タイトル |
Long G2 accumulates recombination intermediates and disturbs chromosome segregation at dysfunction telomere in Schizosaccharomyces pombe.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
Protection of telomere (Pot1) is a single-stranded telomere binding protein which is essential for chromosome ends protection. Fission yeast Rqh1 is a member of RecQ helicases family which has essential roles in the maintenance of genomic stability and regulation of homologous recombination. Double mutant between fission yeast pot1Δ and rqh1 helicase dead (rqh1-hd) maintains telomere by homologous recombination. In pot1Δ rqh1-hd double mutant, recombination intermediates accumulate near telomere which disturb chromosome segregation and make cells sensitive to microtubule inhibitors thiabendazole (TBZ). Deletion of chk1(+) or mutation of its kinase domain shortens the G2 of pot1Δ rqh1-hd double mutant and suppresses both the accumulation of recombination intermediates and the TBZ sensitivity of that double mutant. In this study, we asked whether the long G2 is the reason for the TBZ sensitivity of pot1Δ rqh1-hd double mutant. We found that shortening the G2 of pot1Δ rqh1-hd double mutant by additional mutations of wee1 and mik1 or gain of function mutation of Cdc2 suppresses both the accumulation of recombination intermediates and the TBZ sensitivity of pot1Δ rqh1-hd double mutant. Our results suggest that long G2 of pot1Δ rqh1-hd double mutant may allow time for the accumulation of recombination intermediates which disturb chromosome segregation and make cells sensitive to TBZ.
|
巻・号 |
464(1)
|
ページ |
140-6
|
公開日 |
2015-8-14
|
DOI |
10.1016/j.bbrc.2015.06.098
|
PII |
S0006-291X(15)30150-9
|
PMID |
26093291
|
MeSH |
Binding Sites
CDC2 Protein Kinase / genetics
CDC2 Protein Kinase / metabolism
Cell Cycle Proteins / genetics
Cell Cycle Proteins / metabolism
Checkpoint Kinase 1
Chromosome Segregation / drug effects*
Chromosomes, Fungal / chemistry
Chromosomes, Fungal / drug effects
Chromosomes, Fungal / metabolism
DNA Helicases / genetics
DNA Helicases / metabolism
G2 Phase / drug effects
G2 Phase / genetics*
Gene Expression Regulation, Fungal*
Genome, Fungal*
Genomic Instability
Homologous Recombination / drug effects*
Microbial Sensitivity Tests
Microtubules / chemistry
Microtubules / drug effects
Microtubules / metabolism
Mutation
Nuclear Proteins / genetics
Nuclear Proteins / metabolism
Protein Binding
Protein Kinases / deficiency
Protein Kinases / genetics
Protein-Tyrosine Kinases / genetics
Protein-Tyrosine Kinases / metabolism
Schizosaccharomyces / drug effects
Schizosaccharomyces / genetics*
Schizosaccharomyces / growth & development
Schizosaccharomyces pombe Proteins / genetics
Schizosaccharomyces pombe Proteins / metabolism
Shelterin Complex
Telomere / chemistry
Telomere / drug effects
Telomere / metabolism*
Telomere-Binding Proteins / genetics
Telomere-Binding Proteins / metabolism
Thiabendazole / pharmacology
Tubulin Modulators / pharmacology
|
IF |
2.985
|
引用数 |
0
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
BIOPHYSICS
|
リソース情報 |
酵母 |
FY16194
MY444 |