RRC ID 31349
著者 Wada T, Kunihiro A, Tominaga-Wada R.
タイトル Arabidopsis CAPRICE (MYB) and GLABRA3 (bHLH) control tomato (Solanum lycopersicum) anthocyanin biosynthesis.
ジャーナル PLoS One
Abstract In Arabidopsis thaliana the MYB transcription factor CAPRICE (CPC) and the bHLH transcription factor GLABRA3 (GL3) are central regulators of root-hair differentiation and trichome initiation. By transforming the orthologous tomato genes SlTRY (CPC) and SlGL3 (GL3) into Arabidopsis, we demonstrated that these genes influence epidermal cell differentiation in Arabidopsis, suggesting that tomato and Arabidopsis partially use similar transcription factors for epidermal cell differentiation. CPC and GL3 are also known to be involved in anthocyanin biosynthesis. After transformation into tomato, 35S::CPC inhibited anthocyanin accumulation, whereas GL3::GL3 enhanced anthocyanin accumulation. Real-time reverse transcription PCR analyses showed that the expression of anthocyanin biosynthetic genes including Phe-ammonia lyase (PAL), the flavonoid pathway genes chalcone synthase (CHS), dihydroflavonol reductase (DFR), and anthocyanidin synthase (ANS) were repressed in 35S::CPC tomato. In contrast, the expression levels of PAL, CHS, DFR, and ANS were significantly higher in GL3::GL3 tomato compared with control plants. These results suggest that CPC and GL3 also influence anthocyanin pigment synthesis in tomato.
巻・号 9(9)
ページ e109093
公開日 2014-1-1
DOI 10.1371/journal.pone.0109093
PII PONE-D-14-32195
PMID 25268379
PMC PMC4182634
MeSH Acyltransferases / genetics Acyltransferases / metabolism Alcohol Oxidoreductases / genetics Alcohol Oxidoreductases / metabolism Anthocyanins / biosynthesis* Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Basic Helix-Loop-Helix Transcription Factors / genetics* Basic Helix-Loop-Helix Transcription Factors / metabolism Cell Differentiation Gene Expression Regulation, Plant* Oxygenases / genetics Oxygenases / metabolism Phenylalanine Ammonia-Lyase / genetics Phenylalanine Ammonia-Lyase / metabolism Plant Epidermis / cytology Plant Epidermis / metabolism Plants, Genetically Modified Proto-Oncogene Proteins c-myb / genetics* Proto-Oncogene Proteins c-myb / metabolism Sequence Homology, Amino Acid Solanum lycopersicum / genetics* Solanum lycopersicum / metabolism Transgenes
IF 2.74
引用数 22
WOS 分野 PLANT SCIENCES
リソース情報
トマト TOMJPF00001