論文 - 詳細
RRC ID | 45713 |
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著者 | Cox-Paulson E, Cannataro V, Gallagher T, Hoffman C, Mantione G, Mcintosh M, Silva M, Vissichelli N, Walker R, Simske J, Ono S, Hoops H. |
タイトル | The minus-end actin capping protein, UNC-94/tropomodulin, regulates development of the Caenorhabditis elegans intestine. |
ジャーナル | Dev Dyn |
Abstract |
BACKGROUND:Tropomodulins are actin-capping proteins that regulate the stability of the slow-growing, minus-ends of actin filaments. The C. elegans tropomodulin homolog, UNC-94, has sequence and functional similarity to vertebrate tropomodulins. We investigated the role of UNC-94 in C. elegans intestinal morphogenesis. RESULTS:In the embryonic C. elegans intestine, UNC-94 localizes to the terminal web, an actin- and intermediate filament-rich structure that underlies the apical membrane. Loss of UNC-94 function results in areas of flattened intestinal lumen. In worms homozygous for the strong loss-of-function allele, unc-94(tm724), the terminal web is thinner and the amount of F-actin is reduced, pointing to a role for UNC-94 in regulating the structure of the terminal web. The non-muscle myosin, NMY-1, also localizes to the terminal web, and we present evidence that increasing actomyosin contractility by depleting the myosin phosphatase regulatory subunit, mel-11, can rescue the flattened lumen phenotype of unc-94 mutants. CONCLUSIONS:The data support a model in which minus-end actin capping by UNC-94 promotes proper F-actin structure and contraction in the terminal web, yielding proper shape of the intestinal lumen. This establishes a new role for a tropomodulin in regulating lumen shape during tubulogenesis. |
巻・号 | 243(6) |
ページ | 753-64 |
公開日 | 2014-6-1 |
DOI | 10.1002/dvdy.24118 |
PMID | 24677443 |
PMC | PMC4166613 |
MeSH | Actins / genetics Actins / metabolism Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Embryo, Nonmammalian / cytology Embryo, Nonmammalian / embryology* Intestines / cytology Intestines / embryology* Mutation Myosin Heavy Chains / genetics Myosin Heavy Chains / metabolism Tropomodulin / genetics Tropomodulin / metabolism* |
IF | 3.275 |
引用数 | 1 |
WOS 分野 | ANATOMY & MORPHOLOGY DEVELOPMENTAL BIOLOGY |
リソース情報 | |
線虫 | tm724 |