論文 - 詳細
RRC ID | 46317 |
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著者 | Philogene MC, Small SG, Wang P, Corsi AK. |
タイトル | Distinct Caenorhabditis elegans HLH-8/twist-containing dimers function in the mesoderm. |
ジャーナル | Dev Dyn |
Abstract |
BACKGROUND:The Caenorhabditis elegans basic helix-loop-helix (bHLH) factor HLH-8, the single Twist ortholog in the nematode genome, plays important roles in mesoderm development, including M lineage patterning and differentiation of vulval and enteric muscles. HLH-8 cooperates with HLH-2, the bHLH E/Daughterless ortholog, to regulate downstream target genes, but it is not known whether HLH-2 is an obligate partner for all HLH-8 functions. RESULTS:Using hlh-2 loss-of-function alleles and RNAi, we discovered that HLH-2 is required in the vulval muscles but not in M patterning or enteric muscle development. Additionally, we found that expressing tethered HLH-8/HLH-8 dimers in hlh-8 null animals rescued M patterning and enteric but not vulval muscle development. CONCLUSIONS:These results support a model whereby HLH-8/HLH-8 homodimers function in M lineage patterning and enteric muscles and HLH-8/HLH-2 heterodimers function in the M-derived vulval muscles. Interestingly, the different dimers function in the same M lineage cells and the switch in dimer function coincides with vulval muscle differentiation. The use of distinct Twist dimers is evolutionarily conserved, and C. elegans provides a paradigm for future dissection of differential promoter regulation by these dimers at a single cell resolution. |
巻・号 | 241(3) |
ページ | 481-92 |
公開日 | 2012-3-1 |
DOI | 10.1002/dvdy.23734 |
PMID | 22275075 |
PMC | PMC5515552 |
MeSH | Animals Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism Body Patterning Caenorhabditis elegans / embryology* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Female Intestinal Mucosa / metabolism Male Mesoderm / cytology Mesoderm / metabolism* Muscle, Smooth / embryology* Muscle, Smooth / metabolism* Organ Specificity Protein Multimerization Twist-Related Protein 1 / genetics Twist-Related Protein 1 / metabolism* Vulva / embryology Vulva / physiology |
IF | 3.275 |
引用数 | 6 |
WOS 分野 | ANATOMY & MORPHOLOGY DEVELOPMENTAL BIOLOGY |
リソース情報 | |
線虫 | tm1768 |