RRC ID 52641
著者 He Q, Okajima T, Onoe H, Subagyo A, Sueoka K, Kuribayashi-Shigetomi K.
タイトル Origami-based self-folding of co-cultured NIH/3T3 and HepG2 cells into 3D microstructures.
ジャーナル Sci Rep
Abstract This paper describes an origami-inspired self-folding method to form three-dimensional (3D) microstructures of co-cultured cells. After a confluent monolayer of fibroblasts (NIH/3T3 cells) with loaded hepatocytes (HepG2 cells) was cultured onto two-dimensional (2D) microplates, degradation of the alginate sacrificial layer in the system by addition of alginate lyase triggered NIH/3T3 cells to self-fold the microplates around HepG2 cells, and then 3D cell co-culture microstructures were spontaneously formed. Using this method, we can create a large number of 3D cell co-culture microstructures swiftly with ease in the same time. We find that HepG2 cells confined in the 3D cell co-culture microstructures have an ability to enhance the secreted albumin compared to 2D system in a long culture period. The result indicates that the origami-based cell self-folding technique presented here is useful in regenerative medicine and the preclinical stage of drug development.
巻・号 8(1)
ページ 4556
公開日 2018-3-14
DOI 10.1038/s41598-018-22598-x
PII 10.1038/s41598-018-22598-x
PMID 29540810
PMC PMC5852161
MeSH Animals Cell Shape Cell Survival Coculture Techniques / instrumentation* Coculture Techniques / methods Hep G2 Cells Humans Mice NIH 3T3 Cells Regenerative Medicine Serum Albumin, Human / metabolism*
IF 3.998
引用数 11
リソース情報
ヒト・動物細胞 NIH/3T3(RCB2767) Hep G2(RCB1886)