RRC ID 19674
著者 Yoshida R, Sasakura Y.
タイトル Establishment of enhancer detection lines expressing GFP in the gut of the ascidian Ciona intestinalis.
ジャーナル Zoolog Sci
Abstract The gut is a tubular, endodermal organ for digesting food and absorbing nutrients. In this study, we characterized eight enhancer detection lines that express green fluorescent protein (GFP) in the whole or part of the digestive tube of the ascidian Ciona intestinalis. Three enhancer detection lines for the pyloric gland, a structure associated with the digestive tube, were also analyzed. These lines are valuable markers for analyzing the mechanisms of development of the gut. Based on the GFP expression of the enhancer detection lines together with morphological characteristics, the digestive tube of Ciona can be subdivided into at least 10 compartments in which different genetic cascades operate. Causal insertion sites of the enhancer detection lines were identified, and the expression pattern of the genes near the insertion sites were characterized by means of whole-mount in situ hybridization. We have characterized four and two genes that were specifically or strongly expressed in the digestive tube and pyloric gland, respectively. The present data provide the basic information and useful resources for studying gut formation in Ciona.
巻・号 29(1)
ページ 11-20
公開日 2012-1-1
DOI 10.2108/zsj.29.11
PMID 22233491
MeSH Animals Animals, Genetically Modified Ciona intestinalis / genetics Ciona intestinalis / metabolism* DNA Transposable Elements Enhancer Elements, Genetic / physiology* Gastrointestinal Tract / metabolism* Gene Expression Regulation, Developmental / physiology* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism* Mutagenesis, Insertional
IF 0.843
引用数 14
WOS 分野 ZOOLOGY
リソース情報
カタユウレイボヤ・(ニッポンウミシダ) Mu[MiTSAdTPOG]8 EJ[MiTSAdTPOG]123 EJ[MiTSAdTPOG]109 EJ[MiTSAdTPOG]107 EJ[MiTSAdTPOG]101 EJ[MiTSAdTPOG]92 EJ[MiTSAdTPOG]87 EJ[MiTSAdTPOG]75 EJ[MiTSAdTPOG]71 EJ[MiTSAdTPOG]47-2 EJ[MiTSAdTPOG]36-2 E[MiTSAdTPOG]1 Wild C. int