RRC ID 22817
著者 Matsuda Y, Sahara K, Yasukochi Y, Yamashiki N.
タイトル Detection of gamma-tubulin in spermatogonial cells of Bombyx mori (Lepidoptera) and Chortophaga viridifasciata (Orthoptera).
ジャーナル Zoolog Sci
Abstract We detected a putative gamma-tubulin gene in silico and detected BACs containing the gene from a Bombyx mori BAC library. BAC-FISH mapping revealed that the gene is located on chromosome 5. To observe the distribution of gamma-tubulin, we employed antibodies against mammalian gamma-tubulin peptides. Western blot analysis disclosed a band very similar in size to gamma-tubulin protein in other species (approximately 48 kDa). In mitotic metaphase of B. mori spermatogonial cells, gamma-tubulin is exclusively localized in the spindle poles, where the centrosomes occur. We applied the same system to the grasshopper Chortophaga viridifasciata, as a representative of insect orders in which the gamma-tubulin distribution had not previously been studied. Gamma-tubulin was also found in the spindle poles during metaphase of spermatogonial cells in the grasshopper.
巻・号 24(8)
ページ 781-6
公開日 2007-8-1
DOI 10.2108/zsj.24.781
PMID 18217484
MeSH Animals Antigens / analysis Blotting, Western / veterinary Bombyx* In Situ Hybridization, Fluorescence / veterinary Male Mitosis / immunology Orthoptera* Species Specificity Spermatozoa / physiology* Spindle Apparatus / chemistry* Tubulin / genetics Tubulin / immunology*
IF 0.843
引用数 1
WOS 分野 ZOOLOGY
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