論文 - 詳細
RRC ID | 22817 |
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著者 | Matsuda Y, Sahara K, Yasukochi Y, Yamashiki N. |
タイトル | Detection of gamma-tubulin in spermatogonial cells of Bombyx mori (Lepidoptera) and Chortophaga viridifasciata (Orthoptera). |
ジャーナル | Zoolog Sci |
Abstract |
We detected a putative gamma-tubulin gene in silico and detected BACs containing the gene from a Bombyx mori BAC library. BAC-FISH mapping revealed that the gene is located on chromosome 5. To observe the distribution of gamma-tubulin, we employed antibodies against mammalian gamma-tubulin peptides. Western blot analysis disclosed a band very similar in size to gamma-tubulin protein in other species (approximately 48 kDa). In mitotic metaphase of B. mori spermatogonial cells, gamma-tubulin is exclusively localized in the spindle poles, where the centrosomes occur. We applied the same system to the grasshopper Chortophaga viridifasciata, as a representative of insect orders in which the gamma-tubulin distribution had not previously been studied. Gamma-tubulin was also found in the spindle poles during metaphase of spermatogonial cells in the grasshopper. |
巻・号 | 24(8) |
ページ | 781-6 |
公開日 | 2007-8-1 |
DOI | 10.2108/zsj.24.781 |
PMID | 18217484 |
MeSH | Animals Antigens / analysis Blotting, Western / veterinary Bombyx* In Situ Hybridization, Fluorescence / veterinary Male Mitosis / immunology Orthoptera* Species Specificity Spermatozoa / physiology* Spindle Apparatus / chemistry* Tubulin / genetics Tubulin / immunology* |
IF | 0.843 |
引用数 | 1 |
WOS 分野 | ZOOLOGY |
リソース情報 | |
カイコ |