RRC ID 33644
著者 Hüsken U, Stickney HL, Gestri G, Bianco IH, Faro A, Young RM, Roussigne M, Hawkins TA, Beretta CA, Brinkmann I, Paolini A, Jacinto R, Albadri S, Dreosti E, Tsalavouta M, Schwarz Q, Cavodeassi F, Barth AK, Wen L, Zhang B, Blader P, Yaksi E, Poggi L, Zigman M, Lin S, Wilson SW, Carl M.
タイトル Tcf7l2 is required for left-right asymmetric differentiation of habenular neurons.
ジャーナル Curr Biol
Abstract BACKGROUND:Although left-right asymmetries are common features of nervous systems, their developmental bases are largely unknown. In the zebrafish epithalamus, dorsal habenular neurons adopt medial (dHbm) and lateral (dHbl) subnuclear character at very different frequencies on the left and right sides. The left-sided parapineal promotes the elaboration of dHbl character in the left habenula, albeit by an unknown mechanism. Likewise, the genetic pathways acting within habenular neurons to control their asymmetric differentiated character are unknown.
RESULTS:In a forward genetic screen for mutations that result in loss of habenular asymmetry, we identified two mutant alleles of tcf7l2, a gene that encodes a transcriptional regulator of Wnt signaling. In tcf7l2 mutants, most neurons on both sides differentiate with dHbl identity. Consequently, the habenulae develop symmetrically, with both sides adopting a pronounced leftward character. Tcf7l2 acts cell automously in nascent equipotential neurons, and on the right side, it promotes dHbm and suppresses dHbl differentiation. On the left, the parapineal prevents this Tcf7l2-dependent process, thereby promoting dHbl differentiation.
CONCLUSIONS:Tcf7l2 is essential for lateralized fate selection by habenular neurons that can differentiate along two alternative pathways, thereby leading to major neural circuit asymmetries.
巻・号 24(19)
ページ 2217-27
公開日 2014-10-6
DOI 10.1016/j.cub.2014.08.006
PII S0960-9822(14)00984-1
PMID 25201686
PMC PMC4194317
MeSH Animals Cell Differentiation* Embryo, Nonmammalian / embryology Embryo, Nonmammalian / physiology Gene Expression Regulation Habenula / cytology Habenula / embryology* Neurons / cytology Neurons / physiology* Signal Transduction Transcription Factor 7-Like 2 Protein / genetics* Transcription Factor 7-Like 2 Protein / metabolism Zebrafish / embryology* Zebrafish / physiology Zebrafish Proteins / genetics* Zebrafish Proteins / metabolism
IF 9.601
引用数 26
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ゼブラフィッシュ Tg (brn3a-hsp70:GFP)rw0110b