RRC ID 46781
著者 Nakayama M, Hosoya K, Shimizu-Imanishi Y, Chibana H, Yaguchi T.
タイトル Development of Rapid Identification and Risk Analysis of Moniliella Spp. in Acidic Processed Foods.
ジャーナル Biocontrol Sci
Abstract The number of spoilage incidents in the food industry attributable to a species of the genus Moniliella has recently increased, but the risk of food spoilage has not yet been evaluated. The purpose of this study was to develop a method to rapidly identify high-risk species and to conduct a risk analysis study of Moniliella spp. Acetic acid resistance of M. acetoabutens and ethanol resistance of M. suaveolens were higher than for other Moniliella species. All examined strains of M. acetoabutens developed a high tolerance to acetic acid by being cultured twice in liquid media containing low concentrations of acetic acid. These findings indicate that M. acetoabutens and M. suaveolens are high-risk species for food spoilage and must be discriminated from other fungi. We developed species-specific primers to identify M. acetoabutens and M. suaveolens using the polymerase chain reaction (PCR) to amplify the D1/D2 domain of 28S rDNA. The PCR using the primer sets designed for M. acetoabutens (Mac_F1/R1) and M. suaveolens (Msu_F1/R1) was specific to the target species and did not detect other fungi involved in food spoilage or environmental contamination. This method is expected to be effective for the monitoring of raw materials and components of the food production process.
巻・号 21(2)
ページ 73-80
公開日 2016-1-1
DOI 10.4265/bio.21.73
PMID 27350425
MeSH Acetic Acid / pharmacology Basidiomycota* / classification Basidiomycota* / drug effects Basidiomycota* / genetics Basidiomycota* / isolation & purification Drug Resistance, Fungal Ethanol / pharmacology Food Contamination / analysis* Food Microbiology* Microbial Sensitivity Tests Phylogeny Risk Assessment
IF 0.833
引用数 1
WOS 分野 BIOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
病原微生物 IFM 40871 IFM 41411 IFM 41604 IFM 42321 IFM 46166 IFM 47483 IFM 47730 IFM 48054 IFM 48407 IFM 48422 IFM 50002 IFM 51214 IFM 52957 IFM 52958 IFM 52962 IFM 52963 IFM 52964 IFM 53168 IFM 53866 IFM 55890 IFM 56020 IFM 57839 IFM 59903 IFM 59904 IFM 59905 IFM 59906 IFM 59907 IFM 60291 IFM 60292 IFM 60293 IFM 60294 IFM 60295 IFM 60296 IFM 60297 IFM 60298 IFM 60299 IFM 60348 IFM 60349 IFM 60350 IFM 60351