RRC ID 49544
著者 Kanemitsu Y, Fujitani M, Fujita Y, Zhang S, Su YQ, Kawahara Y, Yamashita T.
タイトル The RNA-binding protein MARF1 promotes cortical neurogenesis through its RNase activity domain.
ジャーナル Sci Rep
Abstract Cortical neurogenesis is a fundamental process of brain development that is spatiotemporally regulated by both intrinsic and extrinsic cues. Although recent evidence has highlighted the significance of transcription factors in cortical neurogenesis, little is known regarding the role of RNA-binding proteins (RBPs) in the post-transcriptional regulation of cortical neurogenesis. Here, we report that meiosis arrest female 1 (MARF1) is an RBP that is expressed during neuronal differentiation. Cortical neurons expressed the somatic form of MARF1 (sMARF1) but not the oocyte form (oMARF1). sMARF1 was enriched in embryonic brains, and its expression level decreased as brain development progressed. Overexpression of sMARF1 in E12.5 neuronal progenitor cells promoted neuronal differentiation, whereas sMARF1 knockdown decreased neuronal progenitor differentiation in vitro. We also examined the function of sMARF1 in vivo using an in utero electroporation technique. Overexpression of sMARF1 increased neuronal differentiation, whereas knockdown of sMARF1 inhibited differentiation in vivo. Moreover, using an RNase domain deletion mutant of sMARF1, we showed that the RNase domain is required for the effects of sMARF1 on cortical neurogenesis in vitro. Our results further elucidate the mechanisms of post-transcriptional regulation of cortical neurogenesis by RBPs.
巻・号 7(1)
ページ 1155
公開日 2017-4-25
DOI 10.1038/s41598-017-01317-y
PII 10.1038/s41598-017-01317-y
PMID 28442784
PMC PMC5430739
MeSH Animals Cell Cycle Proteins / metabolism* Cell Differentiation Cells, Cultured Cerebral Cortex / embryology* Gene Expression Profiling Mice, Inbred ICR Neurogenesis* Pluripotent Stem Cells / physiology RNA-Binding Proteins / metabolism* Ribonucleases / metabolism*
IF 3.998
引用数 4
リソース情報
遺伝子材料 pCAGIG_sMarf1 (RDB15415).