RRC ID |
29752
|
著者 |
Horiuchi T, Maki H, Sekiguchi M.
|
タイトル |
A new conditional lethal mutator (dnaQ49) in Escherichia coli K12.
|
ジャーナル |
Mol Gen Genet
|
Abstract |
A conditional lethal mutator, dnaQ49, was found in Escherichia coli K12. The dnaQ49 mutation caused stimulation of rifampicin-, nalidixic acid- and streptomycin-resistant mutation frequencies 100 to 2000 fold at 30 degrees C and the frequencies were further increased 50 to 100 fold at 35 degrees C or higher temperatures. Cells carrying dnaQ49 were unable to grow in salt-free L-broth at 44.5 degrees C, and DNA synthesis but not protein synthesis of the cells was suppressed under the restrictive conditions. The dnaQ gene was located at about 5 min on the E. coli linkage map and the order of the genes residing in this region was determined to be ton A-dnaE-metD-dnaQ-pro A.
|
巻・号 |
163(3)
|
ページ |
277-83
|
公開日 |
1978-7-25
|
DOI |
10.1007/BF00271956
|
PMID |
355854
|
MeSH |
Chromosome Mapping
Chromosomes, Bacterial
DNA, Bacterial / biosynthesis*
Drug Resistance, Microbial*
Escherichia coli / genetics*
Genes, Lethal*
Hot Temperature
Mutation*
Nalidixic Acid / pharmacology
Rifampin / pharmacology
Streptomycin / pharmacology
|
リソース情報 |
原核生物(大腸菌) |
ME6085 |