RRC ID |
45661
|
著者 |
Yang J, Fong HT, Xie Z, Tan JW, Inoue T.
|
タイトル |
Direct and positive regulation of Caenorhabditis elegans bed-3 by PRDM1/BLIMP1 ortholog BLMP-1.
|
ジャーナル |
Biochim Biophys Acta
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Abstract |
Caenorhabditis elegans BLMP-1 is the homolog of mammalian PRDM1/BLIMP1 transcriptional repressor and a component of the heterochronic pathway regulating developmental timing. We found that BLMP-1 positively and directly regulates the bed-3 gene required for vulval cell division and molting. blmp-1 mutation or RNAi reduces bed-3 reporter expression and causes phenotype similar to bed-3 mutations. We mapped an enhancer element responsible for bed-3 expression in the vulva and the hypodermis to a 200 bp region in the third intron. Using EMSA, we identified BLMP-1 binding sites within this region. Mutating these sites abolished both in vitro BLMP-1 binding and in vivo enhancer activity. Thus, BLMP-1 directly and positively regulates bed-3 transcription, connecting the heterochronic pathway to regulation of vulval cell division and molting. Recently, C. elegans blmp-1 was found to act as a transcriptional repressor. Thus, C. elegans BLMP-1 acts as a transcriptional activator or a repressor depending on the context.
|
巻・号 |
1849(9)
|
ページ |
1229-36
|
公開日 |
2015-9-1
|
DOI |
10.1016/j.bbagrm.2015.07.012
|
PII |
S1874-9399(15)00164-9
|
PMID |
26234645
|
MeSH |
Animals
Caenorhabditis elegans / genetics*
Caenorhabditis elegans Proteins / genetics*
Enhancer Elements, Genetic
Gene Expression Regulation / physiology*
Introns
Transcription Factors / genetics*
Transgenes
|
IF |
3.411
|
引用数 |
3
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
BIOPHYSICS
|
リソース情報 |
線虫 |
NA |