RRC ID 40036
著者 Bratic A, Clemente P, Calvo-Garrido J, Maffezzini C, Felser A, Wibom R, Wedell A, Freyer C, Wredenberg A.
タイトル Mitochondrial Polyadenylation Is a One-Step Process Required for mRNA Integrity and tRNA Maturation.
ジャーナル PLoS Genet
Abstract Polyadenylation has well characterised roles in RNA turnover and translation in a variety of biological systems. While polyadenylation on mitochondrial transcripts has been suggested to be a two-step process required to complete translational stop codons, its involvement in mitochondrial RNA turnover is less well understood. We studied knockdown and knockout models of the mitochondrial poly(A) polymerase (MTPAP) in Drosophila melanogaster and demonstrate that polyadenylation of mitochondrial mRNAs is exclusively performed by MTPAP. Further, our results show that mitochondrial polyadenylation does not regulate mRNA stability but protects the 3' terminal integrity, and that despite a lack of functioning 3' ends, these trimmed transcripts are translated, suggesting that polyadenylation is not required for mitochondrial translation. Additionally, loss of MTPAP leads to reduced steady-state levels and disturbed maturation of tRNACys, indicating that polyadenylation in mitochondria might be important for the stability and maturation of specific tRNAs.
巻・号 12(5)
ページ e1006028
公開日 2016-5-1
DOI 10.1371/journal.pgen.1006028
PII PGENETICS-D-16-00334
PMID 27176048
PMC PMC4866704
MeSH Animals Codon, Terminator Drosophila melanogaster / genetics* Gene Knockdown Techniques Mitochondria / genetics Mitochondrial Proteins / biosynthesis Mitochondrial Proteins / genetics Polyadenylation / genetics* Protein Biosynthesis / genetics* RNA, Messenger / genetics* RNA, Mitochondrial RNA, Transfer / genetics
IF 5.175
引用数 22
WOS 分野 GENETICS & HEREDITY
リソース情報
ショウジョウバエ 11418R-1