RRC ID 11285
著者 Shibata F, Sahara K, Naito Y, Yasukochi Y.
タイトル Reprobing multicolor FISH preparations in lepidopteran chromosome.
ジャーナル Zoolog Sci
Abstract Multicolor fluorescence in-situ hybridization (FISH) and subsequent reprobing of chromosome preparations increase the number of chromosomes and/or anchor loci on the chromosomes simultaneously identified. Reprobing techniques have been widely applied to chromosomes of vertebrates and plants. We have developed a novel reprobing protocol that utilizes multicolor FISH and bacterial artificial chromosome (BAC) probes to examine chromosome preparations in a model lepidopteran species, the silkworm, Bombyx mori. With standard two-color BAC-FISH, routinely used to map genes on B. mori chromosomes, we could localize only two probes on one preparation, whereas our new protocol combining five-color BAC-FISH and preparation reprobing enabled us to simultaneously map 10 probes, as demonstrated with the Bombyx Z chromosome. The improved BAC-FISH technique will facilitate karyotyping and synteny analysis in Lepidoptera.
巻・号 26(3)
ページ 187-90
公開日 2009-3-1
DOI 10.2108/zsj.26.187
PMID 19341338
MeSH Animals Bombyx / genetics* Chromosome Mapping / methods* Chromosomes / genetics* Chromosomes, Artificial, Bacterial Genetic Linkage In Situ Hybridization, Fluorescence / methods*
IF 0.843
引用数 17
WOS 分野 ZOOLOGY
リソース情報
カイコ NA