RRC ID 21892
著者 Sato T, Ogata J, Niki Y.
タイトル BMP and Hh signaling affects primordial germ cell division in Drosophila.
ジャーナル Zoolog Sci
Abstract The germline is segregated from the remainder of the soma during early embryonic development in metazoan species. In Drosophila, female primordial germ cells (PGCs) continue to proliferate during larval development, and become germline stem cells at the early pupal stage. To elucidate the roles of growth factors in larval PGC division, we examined expression patterns of a bone morphogenetic protein (BMP) growth factor, Decapentaplegic (Dpp), and Hedgehog (Hh), along with factors downstream of each, in the ovary during larval development. Dpp signaling appeared in the ovarian soma from early larval development, and was prominent in the terminal filament cells at late larval stage, whereas Hh appeared in the ovarian soma and PGCs from the third instar larval stage. The number of PGCs decreased when components of these signal transduction pathways were abrogated by RNAi in the PGCs, indicating that both Dpp and Hh signals directly regulate PGC proliferation. Experiments on the up- and down-regulation of Dpp and Hh with a tissue-specific Gal4 driver indicated that Dpp and Hh act as extrinsic and autocrine growth factors. Furthermore, heat-pulse experiments with hs-Gal4 showed that Dpp is active in PGC proliferation throughout larval development, whereas Hh has effects only during late larval development. In addition to Dpp, the reduction of Glass bottom boat (Gbb), another BMP molecule, caused a decrease in the number of PGCs and initiation of larval PGCs differentiation into cystocytes, indicating that Gbb functions to promote PGC division and repress differentiation.
巻・号 27(10)
ページ 804-10
公開日 2010-10-1
DOI 10.2108/zsj.27.804
PMID 20887178
MeSH Animals Cell Proliferation Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / embryology* Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism Female Gene Expression Regulation, Developmental / physiology Germ Cells / cytology* Germ Cells / metabolism* Hedgehog Proteins / genetics Hedgehog Proteins / metabolism* Larva Ovary / metabolism Signal Transduction Transforming Growth Factor beta / genetics Transforming Growth Factor beta / metabolism
IF 0.843
引用数 13
WOS 分野 ZOOLOGY
リソース情報
ショウジョウバエ 5562R-1 5562R-3 12399R-1 12399R-2 4637R-1 4637R-2 2125R-1 2125R-2 9885R-1 9885R-2