RRC ID 48828
著者 Lai SL, Marín-Juez R, Moura PL, Kuenne C, Lai JKH, Tsedeke AT, Guenther S, Looso M, Stainier DY.
タイトル Reciprocal analyses in zebrafish and medaka reveal that harnessing the immune response promotes cardiac regeneration.
ジャーナル Elife
Abstract Zebrafish display a distinct ability to regenerate their heart following injury. However, this ability is not shared by another teleost, the medaka. In order to identify cellular and molecular bases for this difference, we performed comparative transcriptomic analyses following cardiac cryoinjury. This comparison points to major differences in immune cell dynamics between these models. Upon closer examination, we observed delayed and reduced macrophage recruitment in medaka, along with delayed neutrophil clearance. To investigate the role of immune responses in cardiac regeneration, we delayed macrophage recruitment in zebrafish and observed compromised neovascularization, neutrophil clearance, cardiomyocyte proliferation and scar resolution. In contrast, stimulating Toll-like receptor signaling in medaka enhanced immune cell dynamics and promoted neovascularization, neutrophil clearance, cardiomyocyte proliferation and scar resolution. Altogether, these data provide further insight into the complex role of the immune response during regeneration, and serve as a platform to identify and test additional regulators of cardiac repair.
巻・号 6
公開日 2017-6-20
DOI 10.7554/eLife.25605
PII e25605
PMID 28632131
PMC PMC5498136
MeSH Animals Cell Proliferation Gene Expression Profiling Heart Injuries / pathology* Immunity, Cellular* Macrophages / immunology Myocytes, Cardiac / physiology Neutrophils / immunology Oryzias Regeneration* Zebrafish
IF 7.08
引用数 81
リソース情報
メダカ Cab-Tg(zfmlc2 5.1k: DsRed2-nuc) (TG1026)