RRC ID |
31997
|
著者 |
Zheng DM, Bian Z, Furuya N, Oliva Trejo JA, Takeda-Ezaki M, Takahashi K, Hiraoka Y, Mineki R, Taka H, Ikeda S, Komatsu M, Fujimura T, Ueno T, Ezaki J.
|
タイトル |
A treadmill exercise reactivates the signaling of the mammalian target of rapamycin (mTor) in the skeletal muscles of starved mice.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
It has been well established that a starvation-induced decrease in insulin/IGF-I and serum amino acids effectively suppresses the mammalian target of rapamycin (mTor) signaling to induce autophagy, which is a major degradative cellular pathway in skeletal muscles. In this study, we investigated the systematic effects of exercise on the mTor signaling of skeletal muscles. Wild type C57BL/6J mice were starved for 24h under synchronous autophagy induction conditions. Under these conditions, endogenous LC3-II increased, while both S6-kinse and S6 ribosomal protein were dephosphorylated in the skeletal muscles, which indicated mTor inactivation. Using GFP-LC3 transgenic mice, it was also confirmed that fluorescent GFP-LC3 dots in the skeletal muscles increased, including soleus, plantaris, and gastrocnemius, which clearly showed autophagosomal induction. These starved mice were then subjected to a single bout of running on a treadmill (12m/min, 2h, with a lean of 10 degrees). Surprisingly, biochemical analyses revealed that the exercise elicited a decrease in the LC3-II/LC3-I ratio as well as an inversion from the dephosphorylated state to the rephosphorylated state of S6-kinase and ribosomal S6 in these skeletal muscles. Consistently, the GFP-LC3 dots of the skeletal muscles were diminished immediately after the exercise. These results indicated that exercise suppressed starvation-induced autophagy through a reactivation of mTor signaling in the skeletal muscles of these starved mice.
|
巻・号 |
456(1)
|
ページ |
519-26
|
公開日 |
2015-1-2
|
DOI |
10.1016/j.bbrc.2014.11.118
|
PII |
S0006-291X(14)02160-3
|
PMID |
25485704
|
MeSH |
Animals
Autophagy
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microtubule-Associated Proteins / metabolism
Muscle, Skeletal / metabolism
Phosphorylation
Physical Conditioning, Animal*
Ribosomal Protein S6 Kinases / metabolism
Running
Signal Transduction
Starvation
TOR Serine-Threonine Kinases / metabolism*
|
IF |
2.985
|
引用数 |
10
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
実験動物マウス |
RBRC00806 |