RRC ID 41202
著者 Abbas S, Ahmed I, Kudo T, Iqbal M, Lee YJ, Fujiwara T, Ohkuma M.
タイトル A heavy metal tolerant novel bacterium, Bacillus malikii sp. nov., isolated from tannery effluent wastewater.
ジャーナル Antonie Van Leeuwenhoek
Abstract The taxonomic position of a Gram-stain positive and heavy metal tolerant bacterium, designated strain NCCP-662(T), was investigated by polyphasic characterisation. Cells of strain NCCP-662(T) were observed to be rod to filamentous shaped, motile and strictly aerobic, and to grow at 10-50 °C (optimum 30-37 °C) and at pH range of 6-10 (optimum pH 7-8). The strain was found to be able to tolerate 0-12 % NaCl (w/v) and heavy metals (Cr 1200 ppm, Pb 1800 ppm and Cu 1200 ppm) in tryptic soya agar medium. The phylogenetic analysis based on the 16S rRNA gene sequence of strain NCCP-662(T) showed that it belongs to the genus Bacillus and showed high sequence similarity (98.2 and 98.0 %, respectively) with the type strains of Bacillus niabensis 4T19(T) and Bacillus halosaccharovorans E33(T). The chemotaxonomic data showed that the major quinone is MK-7; the predominant cellular fatty acids are anteiso-C15 :0, iso-C14:0, iso-C16:0 and C16:0 and iso-C15:0; the major polar lipids are diphosphatidylglycerol, phosphatidylglycerol along with several unidentified glycolipids, phospholipids and polar lipids. The DNA G+C content was determined to be 36.9 mol%. These data also support the affiliation of strain NCCP-662(T) with the genus Bacillus. The level of DNA-DNA relatedness between strain NCCP-662(T) and B. niabensis JCM 16399(T) was 20.5 ± 0.5 %. On the basis of physiological and biochemical characteristics, phylogenetic analyses and DNA-DNA hybridization data, strain NCCP-662(T) can be clearly differentiated from the validly named Bacillus species and thus represents a new species, for which the name Bacillus malikii sp. nov. is proposed with the type strain NCCP-662(T) (= LMG 28369(T) = DSM 29005(T) = JCM 30192(T)).
巻・号 108(6)
ページ 1319-1330
公開日 2015-12-1
DOI 10.1007/s10482-015-0584-2
PII 10.1007/s10482-015-0584-2
PMID 26362330
MeSH Aerobiosis Bacillus / classification* Bacillus / drug effects Bacillus / genetics Bacillus / isolation & purification* Bacterial Typing Techniques Base Composition Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Drug Resistance, Bacterial* Fatty Acids / analysis Glycolipids / analysis Hydrogen-Ion Concentration Industrial Waste Locomotion Metals, Heavy / toxicity* Molecular Sequence Data Nucleic Acid Hybridization Phospholipids / analysis Phylogeny Quinones / analysis RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sodium Chloride / metabolism Temperature Wastewater / microbiology*
IF 2.674
引用数 5
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 16399 JCM 30192