RRC ID 48131
著者 Ivanova EP, López-Pérez M, Webb HK, Ng HJ, Dang TH, Zhukova NV, Mikhailov VV, Crawford RJ, Rodriguez-Valera F.
タイトル Thalassospira australica sp. nov. isolated from sea water.
ジャーナル Antonie Van Leeuwenhoek
Abstract Two Gram-negative, non-pigmented, motile bacteria were isolated from a sea water sample collected at St. Kilda Beach, Port Philip Bay, Victoria, Australia. The two strains were found to grow between 4 and 40 °C, pH 5-10 and tolerate up to 10 % NaCl. A phylogenetic study, based on a 16S rRNA gene sequence analysis indicated that strains NP 3b2(T) and H 94 belong to the genus Thalassospira. The sequence similarity of the 16S rRNA gene between the two new isolates is 99.8 % and between these strains and all validly named Thalassospira species was found to be in the range of 95-99.4 %. The DNA-DNA relatedness between the two strains was found to be 80.2 %, while relatedness with other validly named species of the genus Thalassospira was between 53 and 65 %. The average nucleotide identity (ANI) and the in silico genome-to-genome distance (GGD) between the two bacteria and T. profundimaris WP0211(T), T. xiamenensis M-5(T), 'T. permensis' NBRC 106175(T) and T. lucentensis QMT2(T) was 76-82 % and 21-25 %, respectively. The results of phylogenetic and genomic analysis, together with physiological and biochemical properties, indicated that the two strains represent a new species of the genus Thalassospira. Based on these data, a new species, Thalassospira australica, is proposed with strain NP 3b2(T) (=KMM 6365(T) = JCM 31222(T)) as the type strain.
巻・号 109(8)
ページ 1091-100
公開日 2016-8-1
DOI 10.1007/s10482-016-0710-9
PII 10.1007/s10482-016-0710-9
PMID 27180097
MeSH Australia Base Composition DNA, Bacterial / genetics DNA, Ribosomal / genetics Fatty Acids / metabolism Nucleic Acid Hybridization Phylogeny RNA, Ribosomal, 16S / genetics Rhodospirillaceae / classification* Rhodospirillaceae / genetics Rhodospirillaceae / isolation & purification* Rhodospirillaceae / metabolism Seawater / microbiology* Sequence Analysis, DNA Species Specificity
IF 2.674
引用数 4
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 31222 JCM 18968 JCM 18969 JCM 18746 JCM 14578 JCM 14850