RRC ID 38594
著者 Kojima K, Shimada T, Nagareda Y, Watanabe M, Ishizaki J, Sai Y, Miyamoto K, Aburada M.
タイトル Preventive effect of geniposide on metabolic disease status in spontaneously obese type 2 diabetic mice and free fatty acid-treated HepG2 cells.
ジャーナル Biol Pharm Bull
Abstract Accumulation of visceral fat induces various symptoms of metabolic syndrome such as insulin resistance and abnormal glucose/lipid metabolism and eventually leads to the onset of ischemic cerebrovascular diseases. Geniposide, which is iridoid glycoside from the fruit of Gardenia jasminoides ELLIS, is recognized as being useful against hyperlipidemia and fatty liver. In order to clarify the effect of geniposide on metabolic disease-based visceral fat accumulation and the relevant molecular mechanism, experiments were performed in spontaneously obese Type 2 diabetic TSOD mice and the free fatty acid-treated HepG2 cells. In the TSOD mice, geniposide showed suppression of body weight and visceral fat accumulation, alleviation of abnormal lipid metabolism and suppression of intrahepatic lipid accumulation. In addition, geniposide alleviated abnormal glucose tolerance and hyperinsulinemia, suggesting that geniposide has an insulin resistance-alleviating effect. Next, in order to investigate the direct effect of geniposide on the liver, the effect on the free fatty acid-treated HepG2 fatty liver model was investigated using genipin, which is the aglycone portion of geniposide. Genipin suppressed the intracellular lipid accumulation caused by the free fatty acid treatment and also significantly increased the intracellular expression of a fatty acid oxidation-related gene (peroxisomal proliferator-activated receptor: PPARα). From these results, it was confirmed that geniposide has an anti-obesity effect, an insulin resistance-alleviating effect and an abnormal lipid metabolism-alleviating effect, and the metabolite genipin shows a direct effect on the liver, inducing expression of a lipid metabolism-related gene as one of its molecular mechanisms.
巻・号 34(10)
ページ 1613-8
公開日 2011-1-1
DOI 10.1248/bpb.34.1613
PII JST.JSTAGE/bpb/34.1613
PMID 21963504
MeSH Adipose Tissue / drug effects Adipose Tissue / metabolism Animals Anti-Obesity Agents / metabolism Anti-Obesity Agents / pharmacology* Anti-Obesity Agents / therapeutic use Body Weight / drug effects Diabetes Mellitus, Type 2 / complications Diabetes Mellitus, Type 2 / drug therapy* Diabetes Mellitus, Type 2 / metabolism Diabetes Mellitus, Type 2 / prevention & control Drug Evaluation, Preclinical Fatty Acids, Nonesterified / metabolism Fatty Liver / etiology Fatty Liver / metabolism Gardenia Glucose Intolerance / complications Glucose Intolerance / metabolism Hep G2 Cells Humans Hyperinsulinism / complications Hyperinsulinism / metabolism Hypoglycemic Agents / metabolism Hypoglycemic Agents / pharmacology* Hypoglycemic Agents / therapeutic use Insulin Resistance Iridoids / metabolism Iridoids / pharmacology* Iridoids / therapeutic use Lipid Metabolism / drug effects Liver / metabolism Male Metabolic Diseases / complications Metabolic Diseases / metabolism Metabolic Diseases / prevention & control Metabolic Syndrome / complications Metabolic Syndrome / drug therapy* Metabolic Syndrome / metabolism Mice Mice, Obese Obesity / complications Obesity / metabolism Phytotherapy Plant Preparations / metabolism Plant Preparations / pharmacology Plant Preparations / therapeutic use
IF 1.863
引用数 60
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞