RRC ID 36634
著者 Svensk E, Devkota R, Ståhlman M, Ranji P, Rauthan M, Magnusson F, Hammarsten S, Johansson M, Borén J, Pilon M.
タイトル Caenorhabditis elegans PAQR-2 and IGLR-2 Protect against Glucose Toxicity by Modulating Membrane Lipid Composition.
ジャーナル PLoS Genet
Abstract In spite of the worldwide impact of diabetes on human health, the mechanisms behind glucose toxicity remain elusive. Here we show that C. elegans mutants lacking paqr-2, the worm homolog of the adiponectin receptors AdipoR1/2, or its newly identified functional partner iglr-2, are glucose intolerant and die in the presence of as little as 20 mM glucose. Using FRAP (Fluorescence Recovery After Photobleaching) on living worms, we found that cultivation in the presence of glucose causes a decrease in membrane fluidity in paqr-2 and iglr-2 mutants and that genetic suppressors of this sensitivity act to restore membrane fluidity by promoting fatty acid desaturation. The essential roles of paqr-2 and iglr-2 in the presence of glucose are completely independent from daf-2 and daf-16, the C. elegans homologs of the insulin receptor and its downstream target FoxO, respectively. Using bimolecular fluorescence complementation, we also show that PAQR-2 and IGLR-2 interact on plasma membranes and thus may act together as a fluidity sensor that controls membrane lipid composition.
巻・号 12(4)
ページ e1005982
公開日 2016-4-1
DOI 10.1371/journal.pgen.1005982
PII PGENETICS-D-15-02971
PMID 27082444
PMC PMC4833288
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Fluorescence Recovery After Photobleaching Forkhead Transcription Factors / genetics Glucose / toxicity* Membrane Fluidity / genetics* Membrane Fluidity / physiology Membrane Lipids / metabolism* Membrane Proteins / genetics* Membrane Proteins / metabolism Receptor, Insulin / genetics
IF 5.175
引用数 25
WOS 分野 GENETICS & HEREDITY
リソース情報
線虫 tm3410