RRC ID 45090
著者 Horiguchi A, Yazaki K, Aoyagi M, Ohnuki Y, Kurosawa H.
タイトル Effective Rho-associated protein kinase inhibitor treatment to dissociate human iPS cells for suspension culture to form embryoid body-like cell aggregates.
ジャーナル J Biosci Bioeng
Abstract Treatment conditions using Y-27632 in the preparation of cell suspension of dissociated human pluripotent stem cells (hiPSCs) were investigated in the context of embryoid body (EB)-like cell aggregates. The effectiveness of a pretreatment with Y-27632 before cell dissociation and that of a Y-27632 treatment during cell dissociation were investigated from the viewpoint of simplicity and robustness. The duration of Y-27632 treatment in the preparation process affected the circularity and agglomeration of dissociated hiPSCs. A single application of pretreatment failed to prevent the onset of blebbing. However, a pretreatment promoted the agglomeration of dissociated hiPSCs when combined with the addition of Y-27632 to cell suspension. Our results indicate that pretreatment enhances the agglomeration potential of dissociated hiPSCs. When cell dissociation was performed in the presence of Y-27632, dissociated hiPSCs possessed the highest circularity and significant agglomerating property. It was shown that treatment with Y-27632 during cell dissociation is a simple and robust method to prepare dissociated hiPSCs for suspension culture to form EB-like cell aggregates.
巻・号 118(5)
ページ 588-92
公開日 2014-11-1
DOI 10.1016/j.jbiosc.2014.04.008
PII S1389-1723(14)00139-X
PMID 24856590
MeSH Amides / pharmacology Cell Aggregation / drug effects* Cell Culture Techniques / methods* Embryoid Bodies / cytology Embryoid Bodies / drug effects* Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / drug effects* Induced Pluripotent Stem Cells / enzymology Protein Kinase Inhibitors / pharmacology* Pyridines / pharmacology rho-Associated Kinases / antagonists & inhibitors*
IF 2.366
引用数 3
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
ヒト・動物細胞 201B7(HPS0063)