RRC ID 48044
著者 Miyazaki S, Kim J, Yamagishi Y, Ishiguro T, Okada Y, Tanno Y, Sakuno T, Watanabe Y.
タイトル Meikin-associated polo-like kinase specifies Bub1 distribution in meiosis I.
ジャーナル Genes Cells
Abstract In meiosis I, sister chromatids are captured by microtubules emanating from the same pole (mono-orientation), and centromeric cohesion is protected throughout anaphase. Shugoshin, which is localized to centromeres depending on the phosphorylation of histone H2A by Bub1 kinase, plays a central role in protecting meiotic cohesin Rec8 from separase cleavage. Another key meiotic kinetochore factor, meikin, may regulate cohesion protection, although the underlying molecular mechanisms remain elusive. Here, we show that fission yeast Moa1 (meikin), which associates stably with CENP-C during meiosis I, recruits Plo1 (polo-like kinase) to the kinetochores and phosphorylates Spc7 (KNL1) to accumulate Bub1. Consequently, in contrast to the transient kinetochore localization of mitotic Bub1, meiotic Bub1 persists at kinetochores until anaphase I. The meiotic Bub1 pool ensures robust Sgo1 (shugoshin) localization and cohesion protection at centromeres by cooperating with heterochromatin protein Swi6, which binds and stabilizes Sgo1. Furthermore, molecular genetic analyses show a hierarchical regulation of centromeric cohesion protection by meikin and shugoshin that is important for establishing meiosis-specific chromosome segregation. We provide evidence that the meiosis-specific Bub1 regulation is conserved in mouse.
巻・号 22(6)
ページ 552-567
公開日 2017-6-1
DOI 10.1111/gtc.12496
PMID 28497540
MeSH Animals Cell Adhesion Cell Cycle Proteins / metabolism* Cells, Cultured Centromere / genetics Centromere / metabolism Chromosomal Proteins, Non-Histone / metabolism Chromosomal Proteins, Non-Histone / physiology Gene Expression Regulation, Fungal* Kinetochores Male Meiosis* Mice Mice, Inbred C57BL Mice, Knockout Microtubules / metabolism Phosphorylation Protein Serine-Threonine Kinases / metabolism* Proto-Oncogene Proteins / metabolism* Schizosaccharomyces / cytology Schizosaccharomyces / growth & development Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / metabolism* Spermatocytes / cytology Spermatocytes / metabolism
IF 1.655
引用数 8
WOS 分野 CELL BIOLOGY GENETICS & HEREDITY
リソース情報
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