RRC ID |
19849
|
著者 |
Kajiwara S, Kakizono T, Saito T, Kondo K, Ohtani T, Nishio N, Nagai S, Misawa N.
|
タイトル |
Isolation and functional identification of a novel cDNA for astaxanthin biosynthesis from Haematococcus pluvialis, and astaxanthin synthesis in Escherichia coli.
|
ジャーナル |
Plant Mol Biol
|
Abstract |
We succeeded in isolating a novel cDNA involved in astaxanthin biosynthesis from the green alga Haematococcus pluvialis, by an expression cloning method using an Escherichia coli transformant as a host that synthesizes beta-carotene due to the Erwinia uredovora carotenoid biosynthesis genes. The cloned cDNA was shown to encode a novel enzyme, beta-carotene ketolase (beta-carotene oxygenase), which converted beta-carotene to canthaxanthin via echinenone, through chromatographic and spectroscopic analysis of the pigments accumulated in an E. coli transformant. This indicates that the encoded enzyme is responsible for the direct conversion of methylene to keto groups, a mechanism that usually requires two different enzymatic reactions proceeding via a hydroxy intermediate. Northern blot analysis showed that the mRNA was synthesized only in the cyst cells of H. pluvialis. E. coli carrying the H. pluvialis cDNA and the E. uredovora genes required for zeaxanthin biosynthesis was also found to synthesize astaxanthin (3S, 3'S), which was identified after purification by a variety of spectroscopic methods.
|
巻・号 |
29(2)
|
ページ |
343-52
|
公開日 |
1995-10-1
|
DOI |
10.1007/BF00043657
|
PMID |
7579184
|
MeSH |
Amino Acid Sequence
Bacterial Proteins / genetics*
Base Sequence
Binding Sites / genetics
Blotting, Northern
Carotenoids / analysis
Carotenoids / biosynthesis*
Carotenoids / metabolism
Chlorophyta / enzymology
Chlorophyta / genetics*
Chromatography, High Pressure Liquid
Circular Dichroism
Conserved Sequence
DNA Mutational Analysis
DNA, Complementary / genetics
Escherichia coli / chemistry
Escherichia coli / genetics
Escherichia coli / metabolism
Gene Library
Molecular Sequence Data
Oxygenases / genetics*
Sequence Analysis, DNA
Sequence Deletion
Sequence Homology, Amino Acid
Xanthophylls
beta Carotene
|
IF |
3.302
|
引用数 |
107
|
WOS 分野
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
藻類 |
NIES-144 |