RRC ID 3084
著者 Zolman BK, Nyberg M, Bartel B.
タイトル IBR3, a novel peroxisomal acyl-CoA dehydrogenase-like protein required for indole-3-butyric acid response.
ジャーナル Plant Mol Biol
Abstract Indole-3-butyric acid (IBA) is an endogenous auxin that acts in Arabidopsis primarily via its conversion to the principal auxin indole-3-acetic acid (IAA). Genetic and biochemical evidence indicates that this conversion is similar to peroxisomal fatty acid beta-oxidation, but the specific enzymes catalyzing IBA beta-oxidation have not been identified. We identified an IBA-response mutant (ibr3) with decreased responses to the inhibitory effects of IBA on root elongation or the stimulatory effects of IBA on lateral root formation. However, ibr3 mutants respond normally to other forms of auxin, including IAA. The mutant seedlings germinate and develop normally, even in the absence of sucrose, suggesting that fatty acid beta-oxidation is unaffected. Additionally, double mutants between ibr3 and acx3, which is defective in an acyl-CoA oxidase acting in fatty acid beta-oxidation, have enhanced IBA resistance, consistent with a distinct role for IBR3. Positional cloning revealed that IBR3 encodes a putative acyl-CoA dehydrogenase with a consensus peroxisomal targeting signal. Based on the singular defect of this mutant in responding to IBA, we propose that IBR3 may act directly in the oxidation of IBA to IAA.
巻・号 64(1-2)
ページ 59-72
公開日 2007-5-1
DOI 10.1007/s11103-007-9134-2
PMID 17277896
MeSH Acyl-CoA Dehydrogenase / chemistry Acyl-CoA Dehydrogenase / genetics Acyl-CoA Dehydrogenase / physiology* Acyl-CoA Oxidase / genetics Amino Acid Sequence Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis / metabolism Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / physiology* Butyrates / metabolism Cloning, Molecular Indoleacetic Acids / metabolism Molecular Sequence Data Mutation Peroxisomes / physiology Phylogeny Sequence Alignment
IF 3.302
引用数 59
WOS 分野 PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda08299